| Author | Carvalho, C. R. | |
| Author | Lenzi, Henrique Leonel | |
| Author | Correa-Oliveira, R. | |
| Author | Vaz, N. M. | |
| Access date | 2012-05-15T15:43:46Z | |
| Available date | 2012-05-15T15:43:46Z | |
| Document date | 2002 | |
| Citation | CARVALHO, C. R. et al. Indirect effects of oral tolerance to ovalbumin interfere with the immune responses triggered by Schistosoma mansoni eggs. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v.35, n.10, p.1195-1199, oct. 2002. | pt_BR |
| ISSN | 1414-431X | |
| URI | https://www.arca.fiocruz.br/handle/icict/4071 | |
| Language | eng | pt_BR |
| Rights | open access | |
| Title | Indirect effects of oral tolerance to ovalbumin interfere with the immune responses triggered by Schistosoma mansoni eggs | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0100-879X2002001000012 | |
| Abstract | The objective of the present study was to investigate whether the injection of a tolerated protein (indirect effects) affects the formation of granulomas around Schistosoma mansoni eggs trapped in the lungs after intravenous (iv) injection into normal (noninfected) C57BL/6 mice (6 animals per group). To induce oral tolerance to chicken egg ovalbumin a 1/5 dilution of egg white in water was offered ad libitum in a drinking bottle for 3 days. Control mice received water. After 7 days, control and experimental animals were injected iv with 2,000 S. mansoni eggs through a tail vein. In some mice of both groups the iv injection of eggs was immediately followed by intraperitoneal (ip) immunization with 10 µg of dinitrophenylated conjugates of ovalbumin (DNP-Ova) emulsified in complete Freund's adjuvant (CFA) or only CFA; 18 days later, mice were bled and killed by ether inhalation. The lungs were fixed in formalin and embedded in paraffin. Serial sections of 5 µm were stained with Giemsa, Gomori's silver reticulin and Sirius red (pH 10.2). Granuloma diameters were measured in histological sections previously stained with Gomori's reticulin. Anti-DNP and anti-soluble egg antigen (SEA) antibodies were analyzed by ELISA. In mice orally tolerant to ovalbumin the concomitant ip injection of DNP-Ova resulted in significantly lower anti-SEA antibodies (ELISA*: 1395 ± 352 in non-tolerant and 462 ± 146 in tolerant mice) and affected granuloma formation around eggs, significantly decreasing granuloma size (area: 22,260 ± 2478 to 12,993 ± 3242 µm2). Active mechanisms triggered by injection of tolerated antigen (ovalbumin) reduce granuloma formation. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Oral tolerance | pt_BR |
| Subject | Ovalbumin | pt_BR |
| Subject | Granuloma | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| DeCS | Ovalbumina | pt_BR |
| DeCS | Granuloma | pt_BR |
| DeCS | Schistosoma mansoni | pt_BR |
| DeCS | Tolerância Imunológica | pt_BR |
