| Author | Salles, Daniela | pt_BR |
| Author | Cabral, Rosa Estela Caseira | pt_BR |
| Author | Paixão, Júlio César da | pt_BR |
| Author | Almeida, Carlos Eduardo Bonacossa de | pt_BR |
| Author | Seuánez, Héctor N. | pt_BR |
| Author | Cabral-Neto, Januario Bispo | pt_BR |
| Access date | 2010-08-23T16:00:32Z | |
| Available date | 2010-08-23T16:00:32Z | |
| Document date | 2007 | |
| Citation | SALLES, Daniela et al. Changes in protein expression due to deleterious mutations in the FA/BRCA pathway. Biochem. Biophys. Res. Commun, New York, v. 364, n. 4, p. 755-760, Dec. 2007. | pt_BR |
| ISSN | 0006-291X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/407 | |
| Language | eng | pt_BR |
| Rights | restricted access | |
| Title | Changes in protein expression due to deleterious mutations in the FA/BRCA pathway | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.bbrc.2007.10.025 | pt_BR |
| Abstract | Inherited deleterious mutations in one of the Fanconi anemia genes lead to a disease, characterized by bone marrow failure, myeloid
leukemia, and hypersensitivity to DNA damage. We identified proteins likely associated to the molecular signaling pathways involved in
DNA repair of interstrand cross-link lesions and in mechanisms of genomic stability mediated by FA/BRCA pathways. We compared
protein maps resolved by bidimensional electrophoresis and analyzed differentially expressed proteins, by mass spectrometry, between
FA complementation group C (FANCC)-deficient cells, and their ectopically corrected counterpart in physiological conditions or after
treatment with MMC. We found six differentially expressed proteins; among them, the checkpoint mediator protein MDC1 whose
expression was disrupted in FANCC/cells. The potential role of differentially expressed proteins in FA phenotype is discussed. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Instituto Nacional do Câncer. Divisão Genética. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Comissão Nacional de Energia Nuclear. Instituto de Radioproteção e Dosimetria. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Genética. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Subject | DNA Repair | |
| Subject | DNA Damage Checkpoint | |
| Subject | Protein Expression | |
| Subject | Genome Stability | |
| DeCS | Proteína BRCA2 - genética | |
| DeCS | Anemia de Fanconi - genética | |
| DeCS | Proteína do Grupo de Complementação C da Anemia de Fanconi - genética | |
| DeCS | Proteínas de Grupos de Complementação da Anemia de Fanconi - genética | |
| DeCS | Regulação da Expressão Gênica - genética | |
| DeCS | Proteínas Nucleares - genética | |
| DeCS | Transdução de Sinal - genética | |
| DeCS | Transativadores - genética | |
