| Author | Moreira, Luciana Souza | |
| Author | Soares, Vinicius Cardoso | |
| Author | Dias, Suelen da Silva Gomes | |
| Author | Bozza, Patrícia T. | |
| Access date | 2020-04-19T20:35:28Z | |
| Available date | 2020-04-19T20:35:28Z | |
| Document date | 2019 | |
| Citation | MOREIRA, Luciana Souza et al. Adipose-derived Mesenchymal Stromal Cells Modulate Lipid Metabolism and Lipid Droplet Biogenesis via AKT/mTOR –PPARγ Signalling in Macrophages. Scientific Reports, v. 9, 20304, p. 1-11, 2019. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40881 | |
| Language | eng | pt_BR |
| Publisher | Nature Research | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Gotículas lipídicas | pt_BR |
| Subject in Portuguese | Macrófagos | pt_BR |
| Subject in Portuguese | Mesenquimato derivado do adiposo Células estromais | pt_BR |
| Subject in Portuguese | Metabolismo | pt_BR |
| Subject in Portuguese | Biogênese | pt_BR |
| Title | Adipose-derived Mesenchymal Stromal Cells Modulate Lipid Metabolism and Lipid Droplet Biogenesis via AKT/mTOR -PPARγ Signalling in Macrophages | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1038/s41598-019-56835-8 | |
| Abstract | Mesenchymal stromal cells (MSCs) are a potential therapy for many chronic inflammatory diseases due to their regenerative, immunologic and anti-inflammatory properties. The two-way dialogue between MSCs and macrophages is crucial to tissue regeneration and repair. Previous research demonstrated that murine adipose-derived MSC conditioned medium (ASCcm) reprograms macrophages to an M2-like phenotype which protects from experimental colitis and sepsis. Here, our focus was to determine the molecular mechanism of lipid droplet biogenesis in macrophages re-educated using ASCcm. Adipose-derived MSC conditioned medium promotes phosphorylation of AKT/mTOR pathway proteins in macrophages. Furthermore, increased expression of PPARγ, lipid droplet biogenesis and PGE2 synthesis were observed in M2-like phenotype macrophages (high expression of arginase 1 and elevated IL-10). Treatment with mTOR inhibitor rapamycin or PPARγ inhibitor GW9662 suppressed lipid droplets and PGE2 secretion. However, these inhibitors had no effect on arginase-1 expression. Rapamycin, but not GW9662, inhibit IL-10 secretion. In conclusion, we demonstrate major effects of ASCcm to reprogram macrophage immunometabolism through mTOR and PPARγ dependent and independent pathways. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Lipid Droplet Biogenesis | pt_BR |
| Subject | Macrophages | pt_BR |
| Subject | Mesenchymal Stromal Cells | pt_BR |
| Subject | Lipid Metabolism | pt_BR |
