| Author | Duque, Thabata L. A. | |
| Author | Siqueira, Mariana S. | |
| Author | Travassos, Leonardo H. | |
| Author | Moreira, Otacilio C. | |
| Author | Bozza, Patrícia T. | |
| Author | Melo, Rossana C. N. | |
| Author | Henriques-Pons, Andrea | |
| Author | Menna-Barreto, Rubem F. S. | |
| Access date | 2020-04-20T14:36:14Z | |
| Available date | 2020-04-20T14:36:14Z | |
| Document date | 2020 | |
| Citation | DUQUE, Thabata L. A. et al. The induction of host cell autophagy triggers defense mechanisms against Trypanosoma cruzi infection in vitro. European Journal of Cell Biology, v. 99, p. 1-10, 2020. | pt_BR |
| ISSN | 0171-9335 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40885 | |
| Description | Acesso aberto em 20/04/2020 - hhtp;?https://reader.elsevier.com/reader/sd/pii/S017193351930144X?token=213749C9F9F6F455F65F32295E5545DA7946D0D5020A7B705D38BCBC1BBA38A627A33F1420BBF85ABEA98C394F096A45 | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Autofagia | pt_BR |
| Subject in Portuguese | Inanição | pt_BR |
| Subject in Portuguese | Célula cardíaca | pt_BR |
| Subject in Portuguese | Interação da célula hospedeira | pt_BR |
| Subject in Portuguese | Macrófagos | pt_BR |
| Title | The induction of host cell autophagy triggers defense mechanisms against Trypanosoma cruzi infection in vitro | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.ejcb.2019.151060 | |
| Abstract | Trypanosoma cruzi causes Chagas disease, a neglected illness that affects millions of people worldwide, especially in Latin America. The balance between biochemical pathways triggered by the parasite and host cells response will ultimately define the progression of a life-threatening disease, justifying the efforts to understand cellular mechanisms for infection restrain. In this interaction, parasite and host cells are affected by different physiological responses as autophagy modulation, which could be under intense cellular stress, such as nutrient deprivation, hormone depletion, or infection. Autophagy is a constitutive pathway that leads to degradation of macromolecules and cellular structures and may induce cell death. In Trypanosoma cruzi infection, the relevance of host autophagy is controversial regarding in vitro parasite intracellular life cycle. In the present study, we evaluated host cell autophagy during T. cruzi infection in phagocytic and non-professional phagocytic cells. We described that the presence of the parasite increased the number of LC3 puncta, a marker for autophagy, in cardiac cells and peritoneal macrophages in vitro. The induction of host autophagy decreased infection in macrophages in early and late time-periods. We suggest that starved phagocytic cells reduced internalization, also confirmed by inert particles and dead trypomastigotes. Whereas, in cardiac cells, starvation-induced autophagy decreased lipid droplets and infection in later time-point, by reducing parasite differentiation/proliferation. In ATG5 knockout MEF cells, we confirmed our hypothesis of autophagy machinery activation during parasite internalization, increasing infection. Our data suggest that host autophagy downregulates T. cruzi infection through impairing parasite intracellular life cycle, reducing the infection in primary culture cells. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunoreceptores e Sinalização. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunoreceptores e Sinalização. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Autophagy | pt_BR |
| Subject | Starvation | pt_BR |
| Subject | Cardiac cell | pt_BR |
| Subject | Macrophage | pt_BR |
| Subject | Host cell interaction | pt_BR |
| e-ISSN | 1618-1298 | |
| Embargo date | 2025-01-01 | |
