| Author | Pacheco, Patricia | |
| Author | Abreu, Adriana Vieira de | |
| Author | Gomes, Rachel N. | |
| Author | LIma, Giselle Barbosa | |
| Author | Wermelinger, Leticia B. | |
| Author | Monteiro, Clarissa M. Maya | |
| Author | Silva, Adriana R. | |
| Author | Bozza, Marcelo T. | |
| Author | Faria Neto, Hugo Caire C. | |
| Author | Melo, Christianne Bandeira | |
| Author | Bozza, Patrícia T. | |
| Access date | 2020-04-20T17:26:35Z | |
| Available date | 2020-04-20T17:26:35Z | |
| Document date | 2009 | |
| Citation | PACHECO, Patricia et al. Lipid droplet formation in leprosy: Toll-like receptor-regulated organelles involved in eicosanoid formation and Mycobacterium leprae pathogenesis. Journal of Immunology, p. 1-10, 2020. | pt_BR |
| ISSN | 0741-5400 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40891 | |
| Description | Acesso aberto em 20/04/2020 - https://www.jimmunol.org/content/jimmunol/179/12/8500.full.pdf | pt_BR |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Quimiocina da proteína quimioatraente de monócitos | pt_BR |
| Subject in Portuguese | Biogênese | pt_BR |
| Subject in Portuguese | Corpos lipídicos | pt_BR |
| Subject in Portuguese | Macrófagos | pt_BR |
| Subject in Portuguese | Resposta imune inata | pt_BR |
| Title | Lipid droplet formation in leprosy: Toll-like receptor-regulated organelles involved in eicosanoid formation and Mycobacterium leprae pathogenesis | pt_BR |
| Type | Article | |
| DOI | 10.4049/jimmunol.179.12.8500 | |
| Abstract | Lipid bodies (also known as lipid droplets) are emerging as inflammatory organelles with roles in the innate immune response
to infections and inflammatory processes. In this study, we identified MCP-1 as a key endogenous mediator of lipid body
biogenesis in infection-driven inflammatory disorders and we described the cellular mechanisms and signaling pathways
involved in the ability of MCP-1 to regulate the biogenesis and leukotriene B4 (LTB4) synthetic function of lipid bodies. In
vivo assays in MCP-1 / mice revealed that endogenous MCP-1 produced during polymicrobial infection or LPS-driven
inflammatory responses has a critical role on the activation of lipid body-assembling machinery, as well as on empowering
enzymatically these newly formed lipid bodies with LTB4 synthetic function within macrophages. MCP-1 triggered directly
the rapid biogenesis of distinctive LTB4-synthesizing lipid bodies via CCR2-driven ERK- and PI3K-dependent intracellular
signaling in in vitro-stimulated macrophages. Disturbance of microtubule organization by microtubule-active drugs demonstrated that MCP-1-induced lipid body biogenesis also signals through a pathway dependent on microtubular dynamics.
Besides biogenic process, microtubules control LTB4-synthesizing function of MCP-1-elicited lipid bodies, in part by regulating the compartmentalization of key proteins, as adipose differentiation-related protein and 5-lipoxygenase. Therefore,
infection-elicited MCP-1, besides its known CCR2-driven chemotactic function, appears as a key activator of lipid body
biogenic and functional machineries, signaling through a microtubule-dependent manner. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Monocyte Chemoattractant Protein | pt_BR |
| Subject | Chemokine | pt_BR |
| Subject | Biogenesis | pt_BR |
| Subject | Lipid bodies | pt_BR |
| Subject | Innate Immune Response | pt_BR |
| Subject | Macrophage | pt_BR |
| e-ISSN | 1550-6606 | |
