| Author | Teixeira, Leonardo K. | |
| Author | Fonseca, Bruna P. F. | |
| Author | Abreu, Adriana Vieira de | |
| Author | Barboza, Bianca A. | |
| Author | Robbs, Bruno K. | |
| Author | Bozza, Patrícia T. | |
| Author | Viola, João P. B. | |
| Access date | 2020-04-21T14:09:41Z | |
| Available date | 2020-04-21T14:09:41Z | |
| Document date | 2005 | |
| Citation | TEIXEIRA, Leonardo K. et al. IFN- Production by CD8 T Cells Depends on NFAT1 Transcription Factor and Regulates Th Differentiation. Journal of Immunology, v. 175, p. 5931-5939, 2005. | pt_BR |
| ISSN | 0022-1767 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40903 | |
| Language | eng | pt_BR |
| Publisher | American Association of Immunologists | pt_BR |
| Rights | open access | |
| Subject in Portuguese | CD8 T Cells | pt_BR |
| Subject in Portuguese | IFN-y | pt_BR |
| Subject in Portuguese | LInfócitos T | pt_BR |
| Title | IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation | pt_BR |
| Type | Article | |
| DOI | 10.4049/jimmunol.175.9.5931 | |
| Abstract | CD8+ T lymphocytes are excellent sources of IFN-gamma; however, the molecular mechanisms that dictate IFN-gamma expression upon TCR stimulation in these cells are not completely understood. In this study, we evaluated the involvement of NFAT1 in the regulation of IFN-gamma gene expression in murine CD8+ T cells and its relevance during Th differentiation. We show that CD8+, but not CD4+, T cells, represent the very first source of IFN-gamma upon primary T cell activation, and also that the IFN-gamma produced by naive CD8+ T cells may enhance CD4+ Th1 differentiation in vitro. TCR stimulation rapidly induced IFN-gamma expression in CD8+ T lymphocytes in a cyclosporin A-sensitive manner. Evaluation of CD8+ T cells showed that calcium influx alone was sufficient to activate NFAT1 protein, transactivate IFN-gamma gene promoter, and induce IFN-gamma production. In fact, NFAT1-deficient mice demonstrated highly impaired IFN-gamma production by naive CD8+ T lymphocytes, which were totally rescued after retroviral transduction with NFAT1-encoding vectors. Moreover, NFAT1-dependent IFN-gamma production by the CD8+ T cell compartment was crucial to control a Th2-related response in vivo, such as allergic inflammation. Consistently, CD8alpha- as well as IFN-gamma-deficient mice did not mount a Th1 immune response and also developed in vivo allergic inflammation. Our results clearly indicate that IFN-gamma production by CD8+ T cells is dependent of NFAT1 transcription factor and may be an essential regulator of Th immune responses in vivo. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasi | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasi | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasi | pt_BR |
| Subject | CD8 T Cells | pt_BR |
| Subject | IFN-y Production | pt_BR |
| Subject | T lymphocytes | pt_BR |
| e-ISSN | 1550-6606 | |
