| Author | Ozawa, Patricia M. M. | |
| Author | Vieira, Evelyn | |
| Author | Lemos, Débora S. | |
| Author | Souza, Ingrid L. Melo | |
| Author | Zanata, Silvio M. | |
| Author | Pankievicz, Vânia C. | |
| Author | Tuleski, Thalita R. | |
| Author | Souza, Emanuel Maltempi de | |
| Author | Wowk, Pryscilla Fanini | |
| Author | Urban, Cícero de Andrade | |
| Author | Kuroda, Flavia | |
| Author | Lima, Rubens S. | |
| Author | Almeida, Rodrigo C. | |
| Author | Gradia, Daniela F. | |
| Author | Cavalli, Iglenir João | |
| Author | Cavalli, Luciane Regina | |
| Author | Malheiros, Danielle | |
| Author | Ribeiro, Enilze M. S. F. | |
| Access date | 2020-04-21T18:15:49Z | |
| Available date | 2020-04-21T18:15:49Z | |
| Document date | 2020 | |
| Citation | OZAWA, Patricia M. M. et al. Identification of miRNAs enriched in extracellular vesicles derived from serum samples of breast cancer patients. Biomolecules, v. 10, n. 150, p. 1-16, 2020. | pt_BR |
| ISSN | 2218-273X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40910 | |
| Language | por | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | pt_BR |
| Title | Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3390/biom10010150 | |
| Abstract | MicroRNAs derived from extracellular vesicles (EV-miRNAs) are circulating miRNAs considered as potential new diagnostic markers for cancer that can be easily detected in liquid biopsies. In this study, we performed RNA sequencing analysis as a screening strategy to identify EV-miRNAs derived from serum of clinically well-annotated breast cancer (BC) patients from the south of Brazil. EVs from three groups of samples (healthy controls (CT), luminal A (LA), and triple-negative (TNBC)) were isolated from serum using a precipitation method and analyzed by RNA-seq (screening phase). Subsequently, four EV-miRNAs (miR-142-5p, miR-150-5p, miR-320a, and miR-4433b-5p) were selected to be quantified by quantitative real-time PCR (RT-qPCR) in individual samples (test phase). A panel composed of miR-142-5p, miR-320a, and miR-4433b-5p distinguished BC patients from CT with an area under the curve (AUC) of 0.8387 (93.33% sensitivity, 68.75% specificity). The combination of miR-142-5p and miR-320a distinguished LA patients from CT with an AUC of 0.9410 (100% sensitivity, 93.80% specificity). Interestingly, decreased expression of miR-142-5p and miR-150-5p were significantly associated with more advanced tumor grades (grade III), while the decreased expression of miR-142-5p and miR-320a was associated with a larger tumor size. These results provide insights into the potential application of EVs-miRNAs from serum as novel specific markers for early diagnosis of BC. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Biologia Celular e Molecular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Biologia Celular e Molecular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Positivo. Faculdade de Medicina. Curitiba, PR, Brasil. / Hospital Nossa Senhora das Graças. Unidade de Mama. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Hospital Nossa Senhora das Graças. Unidade de Mama. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Hospital Nossa Senhora das Graças. Unidade de Mama. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. / Department of Biomedical Data Sciences. Molecular Epidemiology. Leiden University Medical Center. Leiden, The Netherlands. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Department of Oncology, Lombardi Comprehensive Cancer Center. Georgetown University. Washington, DC. USA. / Instituto de Pesquisa Pelé Pequeno Príncipe. Faculdades Pequeno Príncipe, Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. | pt_BR |
| Subject | Extracellular Vesicles | pt_BR |
| Subject | Base Sequence | pt_BR |
| Subject | Liquid Biopsy | pt_BR |
| Subject | Breast Neoplasms | pt_BR |
| Subject in Spanish | MicroARNs | pt_BR |
| Subject in Spanish | Secuencia de Bases | pt_BR |
| Subject in Spanish | Biopsia Líquida | pt_BR |
| Subject in Spanish | Neoplasias de la Mama | pt_BR |
| DeCS | Vesículas Extracelulares | pt_BR |
| DeCS | MicroRNAs | pt_BR |
| DeCS | Sequência de Bases | pt_BR |
| DeCS | Biópsia Líquida | pt_BR |
| DeCS | Neoplasias da Mama | pt_BR |
