Author | Crispim, Myuki Alfaia Esashika | |
Author | Reis, Mônica Nogueira da Guarda | |
Author | Fraiji, Nelson | |
Author | Bello, Gonzalo | |
Author | Stefani, Mariane Martins Araújo | |
Access date | 2020-04-22T17:51:53Z | |
Available date | 2020-04-22T17:51:53Z | |
Document date | 2019 | |
Citation | CRISPIM, Myuki Alfaia Esashika et al. Detection of human immunodeficiency virus Type 1 phylogenetic clusters with multidrug resistance mutations among 2011 to 2017 blood donors from the highly endemic Northern Brazilian Amazon. Transfusion, v. 59, p. 2593-2601, Aug. 2019. | pt_BR |
ISSN | 0041-1132 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/40923 | |
Description | Acesso aberto em 22/04/20210 - https://onlinelibrary.wiley.com/doi/full/10.1111/trf.15347 | pt_BR |
Language | eng | pt_BR |
Publisher | Wiley 12 Months | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Vírus da imunodeficiência humana Tipo 1 | pt_BR |
Subject in Portuguese | Aglomerados filogenéticos | pt_BR |
Subject in Portuguese | Mutações de resistência a multidrogas | pt_BR |
Subject in Portuguese | Doadores de sangue | pt_BR |
Subject in Portuguese | Amazônia brasileira | pt_BR |
Subject in Portuguese | Altamente endêmica | pt_BR |
Title | Detection of human immunodeficiency virus Type 1 phylogenetic clusters with multidrug resistance mutations among 2011 to 2017 blood donors from the highly endemic Northern Brazilian Amazon | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/trf.15347 | |
Abstract | BACKGROUND: This study describes transmitted drug resistance (TDR) in blood donors diagnosed with human immunodeficiency virus Type 1 (HIV-1) infection from 2011 to 2017 in three reference public blood centers from the Northern Brazilian Amazon. STUDY DESIGN AND METHODS: This was a crosssectional study on HIV-positive blood donors from HEMOAM, Manaus, Amazonas, AM (n = 198); HEMERON, Porto Velho, Rondônia, RO (n = 20); and HEMORAIMA, Boa Vista, Roraima, RR (n = 9). HIV-1 pol sequences (protease, reverse transcriptase) were analyzed for drug resistance mutations (DRMs) using the Calibrated Population Resistance tool (Stanford). TDR/DRM clusters were investigated by phylogenetic analysis after removing positions associated with drug resistance of Subtype B sequences from untreated and treated subjects from Northern Brazil. RESULTS: Transmitted drug resistance/DRM in blood donors was 11% (25 of 227), all of them from HEMOAM. Most blood donors with TDR/DRM had multiple and similar DRMs. Nonnucleoside reverse transcriptase inhibitor (NNRTI) mutations predominated (10.1%), followed by nucleoside reverse transcriptase inhibitor (NRTI) mutations (5.3%) and protease inhibitor mutations (0.4%). Dual-class NNRTI/NRTI mutations represented 4.8%. Three highly supported Subtype B monophyletic clades mostly composed by individuals from Amazonas with TDR/DRM mutations were identified. The largest transmission cluster contained 10 sequences, eight from HEMOAM and two sequences described previously (one from a treated subject from Amazonas and the other one from Roraima). This cluster was characterized by NRTI (D67N, T69D, T215S/F/L, K219Q) and NNRTI (K101H, K103 N, G190A) mutations. The other two transmission clades comprised only three and two sequences from HEMOAM sharing the E138A NNRTI mutation. CONCLUSIONS: The identification of transmission
clusters of multidrug-resistant viruses in blood donors from Amazonas highlight the need of continued monitoring of TDR/DRM and the importance of pretreatment genotyping in the highly endemic Amazonas state.Brazilian Amazon. | pt_BR |
Affilliation | Fundação de Hematologia e Hemoterapia do Amazonas. HEMOAM. Manaus, AM, Brasil | pt_BR |
Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da AIDS e da Hanseniase. Goiânia, GO, Brasil. | pt_BR |
Affilliation | Fundação de Hematologia e Hemoterapia do Amazonas. HEMOAM. Manaus, AM, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Laboratório de Imunologia da AIDS e da Hanseniase. Goiânia, GO, Brasil. | pt_BR |
Subject | Human immunodeficiency virus Type 1 | pt_BR |
Subject | Phylogenetic clusters | pt_BR |
Subject | Multidrug resistance mutations | pt_BR |
Subject | Blood donors | pt_BR |
Subject | Northern Brazilian Amazon | pt_BR |
Subject | Highly endemic | pt_BR |
e-ISSN | 1537-2995 | |