| Author | Torrentes-Carvalho, Amanda | |
| Author | Sánchez-Arcila, Juan Camilo | |
| Author | Azamor, Tamiris | |
| Author | Barbosa, Luciana Santos | |
| Author | Hottz, Eugênio Damaceno | |
| Author | Gandini, Mariana | |
| Author | Bozza, Fernando A. | |
| Author | Cunha, Rivaldo Venâncio da | |
| Author | Pinto, Luzia Maria de Oliveira | |
| Author | Damasco, Paulo Vieira | |
| Author | Azeredo, Elzinandes Leal de | |
| Access date | 2020-04-23T13:49:29Z | |
| Available date | 2020-04-23T13:49:29Z | |
| Document date | 2020 | |
| Citation | TORRENTES-CARVALHO, Amanda et al. Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease. Scientific Reports, v. 10, n. 6351, p. 1-13, 14 Apr. 2020. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40932 | |
| Language | eng | pt_BR |
| Publisher | Nature Research | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Apoptose | pt_BR |
| Subject in Portuguese | Leucócitos de sangue mononuclear | pt_BR |
| Subject in Portuguese | Pacientes com HIV | pt_BR |
| Subject in Portuguese | Dengue aguda | pt_BR |
| Title | Apoptosis characterization in mononuclear blood leukocytes of HIV patients during dengue acute disease | en_US |
| Type | Article | |
| DOI | 10.1038/s41598-020-62776-4 | pt_BR |
| Abstract | Dengue virus (DENV) co-circulation in Brazil represents a challenge for treatment and vaccine development. Despite public health impact, the occurrence of coinfections with other viruses is a common event. Increased T cell activation and altered inflammatory response are found during DENV coinfection with Human Immunodeficiency Virus (HIV) impacting HIV-pathogenesis. Even with Antiretroviral therapy (ART), HIV- treated patients had chronic immune activation and lymphocyte apoptosis. However, apoptotic mechanisms have not been investigated during coinfection with DENV. Our attention was attracted to apoptotic cell markers expressions in PBMCs from DENV and DENV/HIV coinfected patients. We found CD4/CD8 ratio inversion in most coinfected patients. CD4 T and CD8 T-cell subsets from DENV and DENV/HIV groups expressed low levels of anti-apoptotic protein Bcl-2. Furthermore, CD8 CD95 double positive cells frequency expressing low levels of Bcl-2 were significantly higher in these patients. Additionally, the density of Bcl-2 on classical monocytes (CD14++CD16-) was significantly lower during DENV infection. Upregulation of pro-apoptotic proteins and anti-apoptotic proteins were found in DENV and DENV/HIV, while catalase, an antioxidant protein, was upregulated mainly in DENV/HIV coinfection. These findings provide evidence of apoptosis triggering during DENV/HIV coinfection, which may contribute to knowledge of immunological response during DENV acute infection in HIV-patients treated with ART. | en |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Imunobiologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Tecnologia Imunológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Mato Grosso do Sul. Faculdade de Medicina. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gafreé & Guinle. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Rio de Janeiro. Hospital Pedro Ernesto. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Apoptosis | pt_BR |
| Subject | Mononuclear blood leukocytes | pt_BR |
| Subject | HIV patients | pt_BR |
| Subject | Dengue acute disease | pt_BR |
