| Author | Oliveira, Iara dos Santos da Silva | |
| Author | Colares, Aracélio Viana | |
| Author | Cardoso, Flávia de Oliveira | |
| Author | Tellis, Carla Junqueira Moragas | |
| Author | Chagas, Maria do Socorro dos Santos | |
| Author | Behrens, Maria Dutra | |
| Author | Calabrese, Kátia da Silva | |
| Author | Souza, Fernando Almeida | |
| Author | Silva, Ana Lúcia Abreu | |
| Access date | 2020-04-23T15:15:30Z | |
| Available date | 2020-04-23T15:15:30Z | |
| Document date | 2019 | |
| Citation | OLIVEIRA, Iara dos Santos da Silva et al. Vernonia polysphaera Baker: Antiinflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells. PLoS ONE, v. 14, n. 12, p. 1-19, 12 Dec. 2019. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40935 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Vernonia polysphaera Baker | pt_BR |
| Subject in Portuguese | Atividade anti-inflamatória | pt_BR |
| Subject in Portuguese | In vivo | pt_BR |
| Subject in Portuguese | Células RAW 264.7 | pt_BR |
| Subject in Portuguese | Efeito inibitório | pt_BR |
| Title | Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0225275 | pt_BR |
| Abstract | Species of the Vernonia genius are widely distributed across the world. In traditional communities, they are commonly used in popular medicine for the treatment of inflammatory diseases. The objective of the present study was to evaluate the anti-inflammatory activity of
Vernonia polysphaera Baker hydroalcoholic extract. A λ-carrageenan-induced paw edema
and peritonitis model was established in BALB/c mice. The in vitro activity of the extract was
measured on LPS-stimulated RAW 264.7 cells. There was no toxic effect on mice or on the
cells treated with the extract. Animals treated with V. polysphaera extract demonstrated inhibition of paw edema in comparison with the untreated animals at all the analyzed doses. In
peritonitis, treatment with the extract at a dose of 500 mg/kg resulted in a lower total leukocyte count in the peritoneal fluid and blood and lower levels of IL-1β, IL-6, TNF-α and PGE-2
than the control group. Cells treated with 50 and 100 μg/mL of the extract exhibited lower
levels of nitrite and pro-inflammatory cytokine production and lower COX-2, NF-κB expression. The V. polysphaera extract demonstrated an anti-inflammatory effect, interfering with
cell migration, reducing pro-inflammatory cytokine levels and COX-2 expression and consequent interference with PGE-2, as well as inhibiting NF-κB transcription. | pt_BR |
| Affilliation | Universidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Centro Universitário Dr. Leão Sampaio. Juazeiro do Norte, CE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Departamento de Produtos Naturais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Departamento de Produtos Naturais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Departamento de Produtos Naturais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Maranhão. Pós-Graduação em Ciência Animal. São Luis, MA, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Maranhão. Pós-Graduação em Ciência Animal. São Luis, MA, Brasil. | pt_BR |
| Subject | Vernonia polysphaera Baker | pt_BR |
| Subject | Antiinflammatory activity | pt_BR |
| Subject | In vivo | pt_BR |
| Subject | RAW 264.7 cells | pt_BR |
| Subject | Inhibitory effect | pt_BR |
