| Author | Henrique-Pons, Andrea | |
| Author | Oliveira, Gabriel M. | |
| Author | Paiva, Mauricio M. | |
| Author | Correa, Alexandre F. S. | |
| Author | Batista, Marcos M. | |
| Author | BIsaggio, Rodrigo C. | |
| Author | Liu, Chau-Ching | |
| Author | Almeida, Vinicius Cotta de | |
| Author | Coutinho, Claudia M. L. M. | |
| Author | Persechini, Pedro M. | |
| Author | Araujo-Jorge, Tania C. | |
| Access date | 2020-04-24T18:34:46Z | |
| Available date | 2020-04-24T18:34:46Z | |
| Document date | 2002 | |
| Citation | HENRIQUES-PONS, Andrea et al. Evidence for a perforin-mediated mechanism controlling cardiac inflammation in Trypasoma cruzi infection. International Journal of Experimental Pathology, v. 83, p. 67-79, 2002. | pt_BR |
| ISSN | 0959-9673 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/40961 | |
| Description | Acesso aberto em 24/04/2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2517672/pdf/iep0083-0067.pdf | pt_BR |
| Language | eng | pt_BR |
| Publisher | Blackwell Science Ltd. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Perforin | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Regulação imunológica | pt_BR |
| Subject in Portuguese | Inflamação | pt_BR |
| Subject in Portuguese | Miocardite | pt_BR |
| Subject in Portuguese | Infecção e morte celular | pt_BR |
| Title | Evidence for a perforim-mediated mechanism controlling cardiac inflamamtion in Trypanosma cruzi infection | pt_BR |
| Type | Article | |
| DOI | 10.1046/j.1365-2613.2002.00215.x | pt_BR |
| Abstract | CD8+ T lymphocytes are considered an important cell population involved in the control of parasitaemia and mortality after Trypanosoma cruzi infection. However, despite recent developments in this field, the mechanism whereby this control is exerted is still not completely understood. Here we have used perforin knockout (−/−) mice infected with Y strain T. cruzi in order to evaluate specifically the participation of the perforin-based cytotoxic pathway in the destruction of cardiomyocytes, cellular inflammatory infiltration, and control of parasitaemia and mortality. We observed that although parasitaemia was equivalent in perforin (+/+) and (−/−) groups, survival rate and spontaneous physical performance were significantly lower in the perforin deficient mice. The cardiac inflammatory cell infiltration, mostly composed of CD8+ cells, was more evident in perforin (−/−) mice. Ultrastructural and immunofluorescence analysis, as well as plasma creatine kinase activity, revealed cardiomyocyte damage and necrosis, more evident in perforin (−/−) mice. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) assays performed in heart samples revealed similar and modest levels of apoptosis in both perforin (+/+) and (−/−) mice. These results indicate that perforin does not play a pivotal role in the control of parasitaemia and direct lysis of cardiomyocytes, but seems to be an important molecule involved in the control of cardiac inflammation and pathology induced by a highly virulent strain of T. cruzi. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunobiofísica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunobiofísica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of Pittsburg School of Medicine. Department of Medicine. Pittsburg, PA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunobiofísica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Perforin | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Immune regulation | pt_BR |
| Subject | Inflammation | pt_BR |
| Subject | Myocarditis | pt_BR |
| Subject | Infection and cell death | pt_BR |
