Author | Silvares, Raquel Rangel | |
Author | Pereira, Evelyn Nunes Goulart da Silva | |
Author | Flores, Edgar Eduardo Ilaquita | |
Author | Rodrigues, Karine Lino | |
Author | Silva, Adriana Ribeiro | |
Author | Albuquerque, Cassiano Felipe Gonçalves de | |
Author | Daliry, Anissa | |
Access date | 2020-04-26T20:17:28Z | |
Available date | 2020-04-26T20:17:28Z | |
Document date | 2019 | |
Citation | SILVARES, Raquel Rangel et al. High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, v. 12, p. 1773-1781, 2019. | pt_BR |
ISSN | 1178-7007 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/40971 | |
Language | eng | pt_BR |
Publisher | Dove Medical Press | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Síndrome metabólica | pt_BR |
Subject in Portuguese | Avançado produtos finais de glicação | pt_BR |
Subject in Portuguese | Piridoxamina | pt_BR |
Subject in Portuguese | Disfunção endotelial renal | pt_BR |
Title | High-fat diet-induced kidney alterations in rats with metabolic syndrome: endothelial dysfunction and decreased antioxidant defense | pt_BR |
Type | Article | pt_BR |
DOI | 10.2147/DMSO.S211253 | |
Abstract | Introduction: This study aimed to investigate changes in renal function and the AGERAGE axis in the kidney of a non-genetic animal model of metabolic syndrome (MetS) induced by high-fat diet (HFD). Additionally, we evaluated the protective effect of pyridoxamine (PM), a vitamin B6 analog with anti-AGE effects, in the context of diet-related renal endothelial dysfunction. Methodology: In Wistar rats, the MetS animal model was induced by 20 or 28 weeks of HFD feeding. When indicated, a subgroup of animals was treated daily with PM (60 mg/kg) for 2 months. Tissue perfusion in renal microcirculation was examined by laser speckle contrast imaging. Oxidative stress was analyzed by thiobarbituric acid reactive species and the inflammatory markers by ELISA (TNF-α and IL-1β). Reverse transcription polymerase chain reaction was used to analyze eNOs, IL-6, vascular cell adhesion molecule (VCAM), NADPH oxidase subunit 47 (N47), catalase, and receptor for AGE (RAGE) gene expression.
Results: Wistar rats fed a HFD showed negligible alteration in renal function, decrease in catalase mRNA transcripts and catalase enzyme activity compared to control (CTL) animals. Increased levels of IL-1β were observed in the kidney of MetS-induced rats. HFD-fed rats exhibited kidney endothelial dysfunction, with no significant differences in basal microvascular blood flow. PM significantly improved kidney vasorelaxation in HFD-fed rats. eNOS, VCAM, and RAGE gene expression and AGE content were not altered in kidneys of HFDinduced MetS rats in comparison to CTLs. Conclusions: Our findings suggest that HFD-induced microvascular dysfunction precedes the decline in renal function, and could be related to antioxidant machinery defects and inflammation activation in the kidney. PM showed a vasoprotective effect, and thus, could be an important contributory factor in ameliorating diet-induced renal damage. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunifarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunifarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Estado do Rio de Janeiro. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Investigação Cardiovascular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Metabolic syndrome | pt_BR |
Subject | Kidney endothelial dysfunction | pt_BR |
Subject | Pyridoxamine | pt_BR |
Subject | Advanced glycation end products | pt_BR |
e-ISSN | 1178-7007 | |