| Author | Pereira, Marcela Helena Gonçalves | |
| Author | Figueiredo, Maria Marta | |
| Author | Queiroz, Camila Pereira | |
| Author | Magalhães, Telcia Vasconcelos Barros | |
| Author | Mafra, Adriana | |
| Author | Diniz, Lilian Martins Oliveira | |
| Author | Costa, Ultimo Libânio da | |
| Author | Gollob, Kenneth J. | |
| Author | Antonelli, Lis Ribeiro do Valle | |
| Author | Santiago, Helton da Costa | |
| Access date | 2020-04-28T14:33:56Z | |
| Available date | 2020-04-28T14:33:56Z | |
| Document date | 2020 | |
| Citation | PEREIRA, Marcela Helena Gonçalves et al. T‐cells producing multiple combinations of IFNγ, TNF and IL10 are associated with mild forms of dengue infection. Immunology, v. 160, p. 90–102, 2020 | pt_BR |
| ISSN | 0019-2805 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41008 | |
| Language | eng | pt_BR |
| Publisher | John Wiley & Sons Ltd | pt_BR |
| Rights | restricted access | pt_BR |
| Title | T‐cells producing multiple combinations of IFNγ, TNF and IL10 are associated with mild forms of dengue infection | pt_BR |
| Type | Article | |
| DOI | 10.1111/imm.13185 | |
| Abstract | Multifunctional interleukin 10 (IL10)+ Th1 cells have been implicated in favorable evolution of many infectious diseases, promoting an efficacious immune response while limiting immunopathology. Here, we investigated the presence of multifunctional CD4+ and CD8+ T-cells that expressed interferon gamma (IFNc), IL10 and tumor necrosis factor (TNF), or its combinations during dengue infection. Peripheral blood mononuclear cells (PBMCs) from outpatients with dengue (mild dengue forms) and hospitalized patients (or patients with dengue with warning signs and severe dengue) were cultured in the presence of envelope (ENV) or NS3 peptide libraries of DENV during critical (hospitalization period) and convalescence phases. The production of IFNc, IL10 and TNF by CD4+ and CD8+ T-cells was assessed by flow cytometry. Our data show that patients with mild dengue, when compared with patients with dengue with warning signs and severe dengue, presented higher frequencies of multifunctional T-cells like NS3-specific IFNc/IL10-producing CD4+ Tcells in critical phase and NS3- and ENV-specific CD8+ T-cells producing IFNc/IL10. In addition, NS3-specific CD8+ T-cells producing high levels of IFNc/TNF and IFNc/TNF/IL10 were also observed in the mild dengue group. We observed that multifunctional T-cells produced higher levels of cytokines as measured by intracellular content when compared with single producer T-cells. Importantly, multifunctional CD4+ and CD8+ T-cells producing IFNc, TNF and IL10 simultaneously displayed positive correlation with platelet levels, suggesting a protective role of this population. The presence of IL10+ Th1 and IL10+ Tc1 multifunctional cells was associated with mild dengue presentation, suggesting that these cells play a role in clinical evolution of dengue infection. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundacão Oswaldo Cruz. Instituto Rene Rachou. . Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Hospital Santa Casa. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundacão Hospitalar do Estado de Minas Gerais. Hospital Metropolitano Odilon Behrens. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina . Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Secretaria Municipal de Saude. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | International Research Center, A. C. Camargo Cancer Center, São Paulo, SP, Brazil | pt_BR |
| Affilliation | Fundacão Oswaldo Cruz. Instituto Rene Rachou. . Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquımica e Imunologia. Belo Horizonte, MG, Brazil/ Center for Immunization Research. Johns Hopkins University. Baltimore, MD, USA . | pt_BR |
| Subject | dengue | pt_BR |
| Subject | IL10+ Tc1 | pt_BR |
| Subject | IL10+Th1 | pt_BR |
| Subject | immunoregulation | pt_BR |
| Subject | multifunctional T-cells | pt_BR |
| Embargo date | 2100-01-01 | |
