| Author | Abreu, Adriana Vieira de | |
| Author | Assis, Edson F. | |
| Author | Gomes, Gleice S. | |
| Author | Faria Neto, Hugo Caire C. | |
| Author | Weller, Peter F. | |
| Author | Melo, Christianne Bandeira | |
| Author | Bozza, Patrícia T. | |
| Access date | 2020-05-03T19:27:09Z | |
| Available date | 2020-05-03T19:27:09Z | |
| Document date | 2005 | |
| Citation | ABREU, Adriana Vieira de et al. Allergic Challenge–Elicited Lipid Bodies Compartmentalize In Vivo Leukotriene C4 Synthesis within Eosinophils. American Journal of Respiratory Cell and Molecular Biology, v. 33, p. 254-261, June 2005. | pt_BR |
| ISSN | 1044-1549 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41075 | |
| Language | eng | pt_BR |
| Publisher | American Thoracic Society | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Alergia | pt_BR |
| Subject in Portuguese | Eosinófilos | pt_BR |
| Subject in Portuguese | Corpos lipídicos | pt_BR |
| Subject in Portuguese | Leukotriene C4 | pt_BR |
| Title | Allergic challenge-elicited lipid bodies compartmentalize in vivo leukotriene C4 synthesis within eosinophils | pt_BR |
| Type | Article | |
| DOI | 10.1165/rcmb.2005-0145OC | |
| Abstract | Eosinophils are an important source of leukotriene (LT)C(4), which can be synthesized within lipid bodies-cytoplasmic organelles where eicosanoid formation may take place. Allergy-driven lipid body formation and function have never been investigated. Here, we studied the in vivo induction and role of lipid bodies within eosinophils recruited to sites of allergic inflammation. Using two murine models of allergic inflammation (asthma and pleurisy), we verified that parallel to the eosinophil influx, allergic challenge also induced lipid body formation within recruited eosinophils. Neutralizing antibodies to eotaxin/CCL11, RANTES/CCL5, or CCR3 partially inhibited lipid body formation within recruited eosinophils in the allergic pleurisy model. Likewise, intrapleural administration of RANTES or eotaxin also induced significant influx of eosinophils loaded with lipid bodies. By immunolabeling, we detected the presence of a key enzyme involved in the leukotriene metabolism-5-lipoxygenase-within eosinophil lipid bodies formed in vivo after allergen challenge. Furthermore, specific immunolocalization of newly formed LTC(4) demonstrated that lipid bodies were the sites of formation of this eicosanoid within infiltrating eosinophils. Therefore, allergic inflammation triggers in vivo formation of new lipid bodies within infiltrating eosinophils, a phenomenon largely mediated by eotaxin/RANTES acting via CCR3 receptors. Such in vivo allergen-driven lipid bodies function as intracellular compartments of LTC(4) synthesis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Harvard Medical School. Department of Medicine. Boston, MA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Allergy | pt_BR |
| Subject | CCR3 | pt_BR |
| Subject | Eosinophils | pt_BR |
| Subject | Lipid bodies | pt_BR |
| Subject | LTC4 | pt_BR |
| e-ISSN | 1535-4989 | |
