| Author | Silva, Tatiana Araújo | |
| Author | Ferreira, Luis Felipe de Carvalho | |
| Author | Pereira, Mirian Claudia de Souza | |
| Author | Calvet, Claudia Magalhães | |
| Access date | 2020-05-04T19:03:50Z | |
| Available date | 2020-05-04T19:03:50Z | |
| Document date | 2019 | |
| Citation | SILVA, Tatiana Araújo et al. Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction. International Journal of Molecular Sciences, v. 20, n. 4836, p. 1-21, 2019. | pt_BR |
| ISSN | 1661-6596 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41093 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | TGF-β | pt_BR |
| Subject in Portuguese | Fibrose cardíaca | pt_BR |
| Subject in Portuguese | Matriz extracelular | pt_BR |
| Subject in Portuguese | Caminhos de sinalização | pt_BR |
| Title | Differential Role of TGF-β in Extracellular Matrix Regulation During Trypanosoma cruzi-Host Cell Interaction | pt_BR |
| Type | Article | |
| DOI | 10.3390/ijms20194836 | |
| Abstract | Transforming growth factor beta (TGF-β) is a determinant for inflammation and fibrosis in cardiac and skeletal muscle in Chagas disease. To determine its regulatory mechanisms, we investigated the response of Trypanosoma cruzi-infected cardiomyocytes (CM), cardiac fibroblasts (CF), and L6E9 skeletal myoblasts to TGF-β. Cultures of CM, CF, and L6E9 were infected with T. cruzi (Y strain) and treated with TGF-β (1-10 ng/mL, 1 h or 48 h). Fibronectin (FN) distribution was analyzed by immunofluorescence and Western blot (WB). Phosphorylated SMAD2 (PS2), phospho-p38 (p-p38), and phospho-c-Jun (p-c-Jun) signaling were evaluated by WB. CF and L6E9 showed an increase in FN from 1 ng/mL of TGF-β, while CM displayed FN modulation only after 10 ng/mL treatment. CF and L6E9 showed higher PS2 levels than CM, while p38 was less stimulated in CF than CM and L6E9. T. cruzi infection resulted in localized FN disorganization in CF and L6E9. T. cruzi induced an increase in FN in CF cultures, mainly in uninfected cells. Infected CF cultures treated with TGF-β showed a reduction in PS2 and an increase in p-p38 and p-c-Jun levels. Our data suggest that p38 and c-Jun pathways may be participating in the fibrosis regulatory process mediated by TGF-β after T. cruzi infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | TGF-β | pt_BR |
| Subject | Heart fibrosis | pt_BR |
| Subject | Extracellular matrix | pt_BR |
| Subject | Signaling pathways | pt_BR |
| Subject | SMAD2 | pt_BR |
| Subject | p38 MAPK | pt_BR |
| Subject | c-Jun | pt_BR |
| e-ISSN | 1422-0067 | |
