| Author | Motta, Fernando Couto | |
| Author | Born, Priscila Silva | |
| Author | Resende, Paola Cristina | |
| Author | Brown, David | |
| Author | Siqueira, Marilda Agudo Mendonça Teixeira de | |
| Access date | 2020-05-05T15:03:24Z | |
| Available date | 2020-05-05T15:03:24Z | |
| Document date | 2019 | |
| Citation | MOTTA, Fernando Couto et al. An Inexpensive and Accurate Reverse Transcription-PCR– Melting Temperature Analysis Assay for Real-Time Influenza Virus B Lineage Discrimination. Journal of Clinical Microbiology, v. 57, n. 12, e00602-19, 9p, Dec. 2019. | pt_BR |
| ISSN | 0095-1137 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41099 | |
| Description | Acesso aberto em 05/05/2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879292/pdf/JCM.00602-19.pdf | pt_BR |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Linhagem Yamagata | pt_BR |
| Subject in Portuguese | Curva de fusão | pt_BR |
| Subject in Portuguese | Temperatura de fusão | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase em Tempo Real | pt_BR |
| Subject in Portuguese | Linhagem Victoria | pt_BR |
| Title | An Inexpensive and Accurate Reverse Transcription-PCR-Melting Temperature Analysis Assay for Real-Time Influenza Virus B Lineage Discrimination | pt_BR |
| Type | Article | |
| DOI | 10.1128/JCM.00602-19 | |
| Abstract | In this work, we describe a SYBR-Green one-step reverse transcription-PCR protocol coupled with a melting temperature analysis (RT-PCR-Tm ), which allows the discrimination of influenza B lineages Yamagata and Victoria. The assay is performed using a regular real-time thermocycler and is based on differences in melting temperature (Tm ) of a 131-bp amplicon, obtained from a conserved region of hemagglutinin gene. A total of 410 samples collected during the 2004, 2008, and 2010-2017 influenza seasons in Brazil were tested, and the lineages were correctly characterized using their melting profiles. The temperature range is significantly different between both lineages throughout the time (Mann-Whitney test; P < 0.0001, confidence interval = 95%), and the Tm is not affected by viral load (Spearman correlation test; r = 0.287, P = 2.245 × 10-9). The simplicity and cost-effectiveness of this protocol make it an option for influenza B lineage surveillance worldwide. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e Sarampo. OMS / NIC. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e Sarampo. OMS / NIC. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e Sarampo. OMS / NIC. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e Sarampo. OMS / NIC. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Vírus Respiratórios e Sarampo. OMS / NIC. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Influenza C | pt_BR |
| Subject | Yamagata lineage | pt_BR |
| Subject | Victoria lineage | pt_BR |
| Subject | Melting temperature | pt_BR |
| Subject | Melting curve | pt_BR |
| Subject | RT-PCR | pt_BR |
| e-ISSN | 1098-660X | |
