Author | Fracaro, Letícia | |
Author | Senegaglia, Alexandra C. | |
Author | Herai, Roberto H. | |
Author | Leitolis, Amanda | |
Author | Boldrini-Leite, Lidiane M. | |
Author | Rebelatto, Carmen L. K. | |
Author | Travers, Paul J. | |
Author | Brofman, Paulo R S | |
Author | Dominguez, Alejandro Correa | |
Access date | 2020-05-12T19:32:40Z | |
Available date | 2020-05-12T19:32:40Z | |
Document date | 2020 | |
Citation | FRACARO, Leticia et al. The Expression Profile of Dental Pulp-Derived Stromal Cells Supports Their Limited Capacity to Differentiate into Adipogenic Cells. International Journal of Molecular Sciences. V.21, n.2753, p. 1-17, 2020. | pt_BR |
ISSN | 1422-0067 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/41209 | |
Language | por | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | DPSCs | pt_BR |
Subject in Portuguese | ADSCs | pt_BR |
Title | The expression profile of dental pulp-derived stromal cells supports their limited capacity to differentiate into adipogenic cells | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/ijms21082753 | |
Abstract | Mesenchymal stromal cells (MSCs) can self-renew, differentiate into specialised cells and have different embryonic origins-ectodermal for dental pulp-derived MSCs (DPSCs) and mesodermal for adipose tissue-derived MSCs (ADSCs). Data on DPSCs adipogenic differentiation potential and timing vary, and the lack of molecular and genetic information prompted us to gain a better understanding of DPSCs adipogenic differentiation potential and gene expression profile. While DPSCs differentiated readily along osteogenic and chondrogenic pathways, after 21 days in two different types of adipogenic induction media, DPSCs cultures did not contain lipid vacuoles and had low expression levels of the adipogenic genes proliferator-activated receptor gamma (PPARG), lipoprotein lipase (LPL) and CCAAT/enhancer-binding protein alpha (CEBPA). To better understand this limitation in adipogenesis, transcriptome analysis in undifferentiated DPSCs was carried out, with the ADSC transcriptome used as a positive control. In total, 14,871 transcripts were common to DPSCs and ADSCs, some were unique (DPSCs: 471, ADSCs: 1032), and 510 were differentially expressed genes. Detailed analyses of overrepresented transcripts showed that DPSCs express genes that inhibit adipogenic differentiation, revealing the possible mechanism for their limited adipogenesis. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Programa de Pós-Graduação em Ciências da Saúde. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Centre for Regenerative Medicine. University of Edinburgh. Edinburgh, Scotland. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Paraná. Faculdade de Medicina. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. | pt_BR |
Subject | Mesenchymal Stem Cells | pt_BR |
Subject | Dental Pulp | pt_BR |
Subject | Adipose Tissue | pt_BR |
Subject | Adipogenesis | pt_BR |
Subject | Transcription Factors | pt_BR |
Subject | Wnt Signaling Pathway | pt_BR |
Subject in Spanish | Células Madre Mesenquimatosas | pt_BR |
Subject in Spanish | Pulpa Dental | pt_BR |
Subject in Spanish | Tejido Adiposo | pt_BR |
Subject in Spanish | Adipogénesis | pt_BR |
Subject in Spanish | Factores de Transcripción | pt_BR |
Subject in Spanish | Vía de Señalización Wnt | pt_BR |
DeCS | Células-Tronco Mesenquimais | pt_BR |
DeCS | Polpa Dentária | pt_BR |
DeCS | Tecido Adiposo | pt_BR |
DeCS | Adipogenia | pt_BR |
DeCS | Fatores de Transcrição | pt_BR |
DeCS | Via de Sinalização Wnt | pt_BR |