| Author | Assis, Patrícia Aparecida | |
| Author | Durso, Danielle Fernandes | |
| Author | Cavalcante, Fernanda Chacon | |
| Author | Zaniratto, Ricardo | |
| Author | Silva, Ana Carolina Carvalho | |
| Author | Cunha Neto, Edecio | |
| Author | Golenbock, Douglas Taylor | |
| Author | Ferreira, Ludmila Rodrigues Pinto | |
| Author | Gazzinelli, Ricardo Tostes | |
| Access date | 2020-05-19T17:38:34Z | |
| Available date | 2020-05-19T17:38:34Z | |
| Document date | 2020 | |
| Citation | ASSIS, Patrícia Aparecida et al. Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria. J Leukoc Biol., 1–15, 2020 | pt_BR |
| ISSN | 2470-0143 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41307 | |
| Language | eng | pt_BR |
| Publisher | American Chemical Society | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Integrative analysis of microRNA and mRNA expression profiles of monocyte-derived dendritic cells differentiation during experimental cerebral malaria. | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1021/acsomega.9b03344 | |
| Abstract | Heterogeneity and high plasticity are common features of cells from the mononuclear phagocyte system: monocytes (MOs), macrophages, and dendritic cells (DCs). Upon activation by microbial agents, MO can differentiate into MO-derived DCs (MODCs). In previous work, we have shown that during acute infection with Plasmodium berghei ANKA (PbA), MODCs become, transiently, the main CD11b+ myeloid population in the spleen (SP) and once recruited to the brain play an important role in the development of experimental cerebral malaria (ECM). Here, we isolated 4 cell populations: bone marrow (BM) MOs (BM-MOs) and SP-MOs from uninfected mice; BM inflammatory MOs (BM-iMOs) and SP-MODCs from PbA-infected mice and used a system biology approach to a holistic transcriptomic comparison and provide an interactome analysis by integrating differentially expressed miRNAs (DEMs) and their differentially expressed gene targets (DEGs) data. The Jaccard index (JI) was used for gauging the similarity and diversity among these cell populations. Whereas BM-MOs, BM-iMOs, and SP-MOs presented high similarity of DEGs, SP-MODCs distinguished by showing a greater number of DEGs. Moreover, functional analysis identified an enrichment in canonical pathways, such as DC maturation, neuroinflammation, and IFN signaling. Upstream regulator analysis identified IFNγ as the potential upstream molecule that can explain the observed DEMs-Target DEGs intersections in SP-MODCs. Finally, directed target analysis and in vivo/ex vivo assays indicate that SP-MODCs differentiate in the SP and IFNγ is a main driver of this process. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia de Sistemas de RNA. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Division of Infectious Diseases and Immunology, University of Massachusetts Medical School. Worcester, Massachusetts, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Faculdade de Medicina. Instituto do Coração. Laboratório de Imunologia. Universidade de São Paulo. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia de Sistemas de RNA. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Faculdade de Medicina. Instituto do Coração. Laboratório de Imunologia. Universidade de São Paulo. São Paulo, SP, Brasil/Universidade de São Paulo. Faculdade de Medicina. Divisão de Imunologia Clínica e Alergia. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Division of Infectious Diseases and Immunology, University of Massachusetts Medical School. Worcester, Massachusetts, USA. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Laboratório de Biologia de Sistemas de RNA. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Division of Infectious Diseases and Immunology. University of Massachusetts Medical School.Worcester,Massachusetts, USA/Fundação Oswaldo Cruz. Instituto Rene Rachou. Laboratorio de Imunopatologia. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Plataforma de Medicina Translacional. /Faculdade de Medicina de Ribeirão Preto. Ribeirão Preto, SP, Brazil | pt_BR |
| Subject | Plasmodium berghei | pt_BR |
| Embargo date | 2035-01-01 | |
