| Author | Marino, Ana Paula Maia Peixoto | |
| Author | Santos, Luara Isabela dos | |
| Author | Henriques, Priscilla M. | |
| Author | Roffe, Ester | |
| Author | Vasconcelos-Santos, Daniel V. | |
| Author | Sher, Alan | |
| Author | Jankovic, Dragana | |
| Author | Gomes, Matheus S. | |
| Author | Amaral, Laurence R. | |
| Author | Azevedo, Ana Carolina Campi | |
| Author | Carvalho, Andréa Teixeira de | |
| Author | Martins Filho, Olindo Assis | |
| Author | Gazzinelli, Ricardo Tostes | |
| Author | Antonelli, Lis Ribeiro | |
| Access date | 2020-06-04T17:41:46Z | |
| Available date | 2020-06-04T17:41:46Z | |
| Document date | 2020 | |
| Citation | MARINO, Ana Paula Maia Peixoto et al. Circulating Inflammatory Mediators as Biomarkers of Ocular Toxoplasmosis in Acute and in Chronic Infection. Journal of Leukocyte Biology, p. 1-12, Apr. 2020. | pt_BR |
| ISSN | 0741-5400 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41531 | |
| Language | por | pt_BR |
| Publisher | Wiley on behalf of the Society for Leukocyte Biology | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Circulating Inflammatory Mediators as Biomarkers of Ocular Toxoplasmosis in Acute and in Chronic Infection | pt_BR |
| Type | Article | |
| DOI | 10.1002/JLB.4MA0420-702R | |
| Abstract | Toxoplasmosis is highly endemic worldwide. In Brazil, depending on the geographical region and socioeconomic status, 40-70% of individuals become seropositive at some point in their lives. A significant proportion of Toxoplasma gondii-chronically infected individuals who are otherwise immunocompetent develop recurrent ocular lesions. The inflammatory/immune mechanisms involved in development of ocular lesion are still unknown and, despite previous investigation, there are no reliable immune biomarkers to predict/follow disease outcome. To better understand the impact of the immune response on parasite control and immunopathology of ocular toxoplasmosis, and to provide insights on putative biomarkers for disease monitoring, we assessed the production of a large panel of circulating immune mediators in a longitudinal study of patients with postnatally acquired toxoplasmosis stratified by the presence of ocular involvement, both at the early acute stage and 6 months later during chronic infection, correlating them with presence of ocular involvement. We found that T. gondii-infected patients, especially during the acute stage of the disease, display high levels of chemokines, cytokines, and growth factors involved in the activation, proliferation, and migration of inflammatory cells to injured tissues. In particular, major increases were found in the IFN-induced chemokines CXCL9 and CXCL10 in T. gondii-infected patients regardless of disease stage or clinical manifestations. Moreover, a specific subgroup of circulating cytokines and chemokines including GM-CSF, CCL25, CCL11, CXCL12, CXCL13, and CCL2 was identified as potential biomarkers that accurately distinguish different stages of infection and predict the occurrence of ocular toxoplasmosis. In addition to serving as predictors of disease development, these host inflammatory molecules may offer promise as candidate targets for therapeutic intervention. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil / Faculdade de Ciências Médicas de Minas Gerais. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil / Laboratory of Molecular Immunology. Molecular Signaling Section. National Institutes of Allergy and Infectious Diseases. National Institutes of Health. Bethesda, Maryland, USA. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Oftalmologia e Otorrinolaringologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Immunobiology Section. Laboratory of Parasitic Diseases. National Institute of Allergy and Infectious Diseases. National Institutes of Health. Bethesda, Maryland, USA. | pt_BR |
| Affilliation | Immunobiology Section. Laboratory of Parasitic Diseases. National Institute of Allergy and Infectious Diseases. National Institutes of Health. Bethesda, Maryland, USA. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Patos de Minas, MG, Brasil / Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Uberlândia Rede Multidisciplinar de Pesquisa, Ciência e Tecnologia. Patos de Minas, MG, Brasil / Universidade Federal de Uberlândia. Laboratório de Bioinformática e Análises Moleculares. Patos de Minas, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Chemokines | pt_BR |
| Subject | Cytokines | pt_BR |
| Subject | Inflammatory response | pt_BR |
| Subject | Toxoplasma gondii | pt_BR |
| Subject | Toxoplasmosis | pt_BR |
| Subject | Uveitis | pt_BR |
| Embargo date | 2035-01-01 | |
