| Author | Castro, Thales Nascimento E. | |
| Author | Costa, Edlaine Rijo | |
| Author | Gonçalves, José Carlos Saraiva | |
| Author | Estrela, Rita de Cassia Elias | |
| Access date | 2020-06-04T20:58:41Z | |
| Available date | 2020-06-04T20:58:41Z | |
| Document date | 2020 | |
| Citation | CASTRO, Thales Nascimento E. et al. Pretreatment and non-specific binding in ultrafiltration device: Impact on protease inhibitor quantification. Journal of Chromatography B, v. 1146, n. 122127, p. 1-5, 2020. | pt_BR |
| ISSN | 1570-0232 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/41544 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Pretreatment and non-specific binding in ultrafiltration device: Impact on protease inhibitor quantification | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jchromb.2020.122127 | |
| Abstract | Introduction: Ultrafiltration (UF) is used to separate unbound drugs; however, non-specific binding (NSB) may be a limiting factor of this technique. Pretreatment of UF devices has been suggested to reduce NSB. Therefore, the pretreatment methodologies for UF devices were evaluated in order to test their effectiveness in reducing NSB of protease inhibitors (PIs).
Methodology: Two PIs (lopinavir-LPV and ritonavir-RTV) were tested. UF devices were pretreated with ultrapure water, Tween-20 or Tween-80. To evaluate the NSB, after UF devices being pretreated, ultrafiltrate solutions containing the analytes at two concentrations (low and high) were used. Samples were quantified by LC-MS/MS.
Results: UF devices pretreated with Tween-5% had the lowest NSB for both analytes. NSB values varied between 7 and 11% at low concentration 16-34% at high LPV concentration, respectively. For RTV, NSB was approximately 6% for low concentration and 18% for high concentration. Failure to completely remove Tween in UF devices could results in an overestimation of NSB.
Conclusion: Pretreatment of UF device with Tween and subsequent removal proved to be effective in reducing NSB of PI. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Programa de Pós-Graduação em Saúde Pública e Meio Ambiente. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Non-specific binding | pt_BR |
| Subject | Ultrafiltration | pt_BR |
| Subject | Unbound drug | pt_BR |
| Subject | Protease inhibitor | pt_BR |
| Embargo date | 2051-01-01 | |
