| Author | Veloso, Laura Barral | |
| Author | Cardoso, Flávia de Oliveira | |
| Author | Charret, Karen dos Santos | |
| Author | Silva, Matheus Pereira de Sá | |
| Author | Côrtes, Luzia Monteiro de Castro | |
| Author | Calabrese, Kátia da Silva | |
| Author | Silva, Franklin Souza da | |
| Author | Sousa, Joel Fontes de | |
| Author | Rocha, Marília Fonseca | |
| Author | Alves, Carlos Roberto | |
| Access date | 2020-07-15T21:04:00Z | |
| Available date | 2020-07-15T21:04:00Z | |
| Document date | 2020 | |
| Citation | VELOSO, Laura Barral et al. Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs. BioMed Research International, v. 2020, Article ID 2615787, 8p, 2020. | pt_BR |
| ISSN | 2314-6133 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42271 | |
| Language | eng | pt_BR |
| Publisher | Hindawi | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Leishmania (Leishmania) infantum | pt_BR |
| Subject in Portuguese | Detecção de RNA | pt_BR |
| Subject in Portuguese | Metaloproteases | pt_BR |
| Subject in Portuguese | Proteases de cisteína | pt_BR |
| Subject in Portuguese | Leishmania (Leishmania) infantum | pt_BR |
| Subject in Portuguese | Infecção | pt_BR |
| Subject in Portuguese | Peles de cães | pt_BR |
| Title | Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs | pt_BR |
| Type | Article | |
| Abstract | Leishmania spp. proteases have been proposed as virulence factors contributing to adaptive success these parasites to the
mammalian hosts. Since these enzymes are poorly studied in naturally infected dogs, this work aims to show the differences in
metalloprotease and cysteine proteases gene expression in ear edge skin of dogs naturally infected by Leishmania (Leishmania)
infantum. A cohort of dogs (n = 20) naturally infected by L. (L.) infantum was clinically classified as asymptomatic,
oligosymptomatic, and polysymptomatic and the parasite load range estimated. The analysis of proteases expression by RT-PCR
in the ear edge skin was also assessed, suggesting more transcripts of proteases in cDNA samples from polysymptomatic dogs
than oligosymptomatic and asymptomatic ones. Metalloprotease RT-PCR assays yielded products (202 bp) in all assessed cDNA
dog samples. In contrast, cysteine proteases transcripts (227 bp) had shown to be better detected in cDNA samples of
polysymptomatic dogs, compared with cDNA samples from asymptomatic and oligosymptomatic dogs. Predictive in silico
assays suggested that secondary structures of metalloproteasee mRNAs can be more stable than cysteine proteases at the skin
temperature of dogs. Evidence is presented that during natural infection of dogs by L. (L.) infantum, this parasite produces
transcripts of metalloprotease and cysteine protease RNA in the skin from asymptomatic, oligosymptomatic, and
polysymptomatic dogs. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Prefeitura Municipal de Montes Claros. Centro de Controle de Zoonoses. Montes Claros, MG, Brasil. | pt_BR |
| Affilliation | Prefeitura Municipal de Montes Claros. Centro de Controle de Zoonoses. Montes Claros, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Leishmania (Leishmania) infantum | pt_BR |
| Subject | Metalloproteases | pt_BR |
| Subject | Cysteine Proteases | pt_BR |
| Subject | Detection | pt_BR |
| Subject | Infected Dogs | pt_BR |
| e-ISSN | 2314-6141 | |
