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AutorNogueira, Mateus Lima
AutorLima, Emilly J. S. P. de
AutorAdrião, Asenate A. X.
AutorFontes, Sheila S.
AutorSilva, Valdenizia Rodrigues
AutorSantos, Luciano de S.
AutorSoares, Milena Botelho Pereira
AutorDias, Rosane Borges
AutorRocha, Clarissa Araújo Gurgel
AutorCosta, Emmanoel Vilaça
AutorSilva, Felipe Moura Araujo da
AutorSantos, Marcos André Vannier
AutorCardozo, Nállarett M. D.
AutorKoolen, Hector Henrique Ferreira
AutorBezerra, Daniel Pereira
Data de acesso2020-07-30T13:37:48Z
Data de disponibilização2020-07-30T13:37:48Z
Data do publicação2020
CitaçãoNOGUEIRA, Mateus Lima et al. Cyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Model. Molecules, v. 25, 2020.pt_BR
ISSN1420-3049pt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/42459
FomentoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM); and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).pt_BR
Idiomaengpt_BR
EditorMDPIpt_BR
Direito Autoralopen accesspt_BR
Palavras-chaveCyperus articulapt_BR
Palavras-chaveMorte celularpt_BR
Palavras-chaveAntitumorpt_BR
Palavras-chaveCarcinoma hepatocelularpt_BR
TítuloCyperus articulatus L. (Cyperaceae) Rhizome Essential Oil Causes Cell Cycle Arrest in the G2/M Phase and Cell Death in HepG2 Cells and Inhibits the Development of Tumors in a Xenograft Modelpt_BR
Tipo do documentoArticlept_BR
DOI10.3390/molecules25112687
Resumo em InglêsCyperus articulatus L. (Cyperaceae), popularly known in Brazil as "priprioca" or "piriprioca", is a tropical and subtropical plant used in popular medical practices to treat many diseases, including cancer. In this study, C. articulatus rhizome essential oil (EO), collected from the Brazilian Amazon rainforest, was addressed in relation to its chemical composition, induction of cell death in vitro and inhibition of tumor development in vivo, using human hepatocellular carcinoma HepG2 cells as a cell model. EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized qualitatively and quantitatively by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detection (GC-FID), respectively. The cytotoxic activity of EO was examined against five cancer cell lines (HepG2, HCT116, MCF-7, HL-60 and B16-F10) and one non-cancerous one (MRC-5) using the Alamar blue assay. Cell cycle distribution and cell death were investigated using flow cytometry in HepG2 cells treated with EO after 24, 48 and 72 h of incubation. The cells were also stained with May-Grunwald-Giemsa to analyze the morphological changes. The anti-liver-cancer activity of EO in vivo was evaluated in C.B-17 severe combined immunodeficient (SCID) mice with HepG2 cell xenografts. The main representative substances of this EO sample were muskatone (11.6%), cyclocolorenone (10.3%), α-pinene (8.26%), pogostol (6.36%), α-copaene (4.83%) and caryophyllene oxide (4.82%). EO showed IC50 values for cancer cell lines ranging from 28.5 µg/mL for HepG2 to >50 µg/mL for HCT116, and an IC50 value for non-cancerous of 46.0 µg/mL (MRC-5), showing selectivity indices below 2-fold for all cancer cells tested. HepG2 cells treated with EO showed cell cycle arrest at G2/M along with internucleosomal DNA fragmentation. The morphological alterations included cell shrinkage and chromatin condensation. Treatment with EO also increased the percentage of apoptotic-like cells. The in vivo tumor mass inhibition rates of EO were 46.5-50.0%. The results obtained indicate the anti-liver-cancer potential of C. articulatus rhizome EO.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.pt_BR
AfiliaçãoAmazonas State University. Metabolomics and Mass Spectrometry Research Group. Amazonas, MA, Brazil.pt_BR
AfiliaçãoAmazonas State University. Metabolomics and Mass Spectrometry Research Group. Amazonas, MA, Brazil.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Bahia, Salvador, BA, Brazil.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Bahia, Salvador, BA, Brazil.pt_BR
AfiliaçãoFederal University of Amazonas. Department of Chemistry. Amazonas, MA, Brazil.pt_BR
AfiliaçãoFederal University of Amazonas. Department of Chemistry. Amazonas, MA, Brazil.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.pt_BR
AfiliaçãoAmazonia Museum. Amazonas, MA, Brazil.pt_BR
AfiliaçãoAmazonas State University. Metabolomics and Mass Spectrometry Research Group. Amazonas, MA, Brazil.pt_BR
AfiliaçãoFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.pt_BR
Palavras-chave em inglêsCyperus articulatespt_BR
Palavras-chave em inglêsCell deathpt_BR
Palavras-chave em inglêsG2/M arrestpt_BR
Palavras-chave em inglêsHepG2pt_BR
Palavras-chave em inglêsAntitumorpt_BR


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