| Author | Lima, Monique da Rocha Queiroz | |
| Author | Carneiro, Thais Chouin | |
| Author | Azeredo, Elzinandes | |
| Author | Barbosa, Luciana Santos | |
| Author | Souza, Thiara Manuele Alves | |
| Author | Silva, Jéssica Badolato Corrêa da | |
| Author | Nunes, Priscila Conrado Guerra | |
| Author | Dal Fabbro, Márcia | |
| Author | Facco, Izilyanne H. Romanholi | |
| Author | Cunha, Rivaldo Venâncio da | |
| Author | Santos, Flavia Barreto dos | |
| Access date | 2020-08-03T15:23:38Z | |
| Available date | 2020-08-03T15:23:38Z | |
| Document date | 2019 | |
| Citation | LIMA, Monique da Rocha Queiroz et al. The inability of a dengue NS1 ELISA to detect Zika infections. Scientifc Reports, v. 9, p. 1-7, 2019. | pt_BR |
| ISSN | 2045-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42508 | |
| Language | eng | pt_BR |
| Publisher | Nature Research | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Incapacidade | pt_BR |
| Subject in Portuguese | ELISA NS1 | pt_BR |
| Subject in Portuguese | Dengue | pt_BR |
| Subject in Portuguese | Infecções | pt_BR |
| Subject in Portuguese | Zika | pt_BR |
| Title | The inability of a dengue NS1 ELISA to detect Zika infections | pt_BR |
| Type | Article | |
| DOI | 10.1038/s41598-019-55160-4 | |
| Abstract | The presence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) in Brazil, may result in a difficult diagnosis due to the signs and symptoms shared by those. Moreover, as DENV and ZIKV belong to the same family, serological assays may show a high rate of cross-reactivity. Here, we evaluated a Dengue NS1 capture assay for early and differential diagnosis of dengue during the Zika epidemic occurred in Brazil in 2016. Samples (n = 227) from 218 patients included sera, plasma and urine from previously confirmed acute cases of Zika, dengue and Zika/dengue co-infections. Nine of those patients presented two specimens. The Dengue NS1 test was very specific for dengue diagnosis (99.32%), even in the co-circulation with ZIKV, and exhibited a high accuracy in not detecting acute Zika infections (92.43%). Our findings showed that the dengue NS1 capture test analyzed here was not able to recognize the ZIKV NS1 and its potential for cross-reaction. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Departamento de Clínica Médica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Departamento de Clínica Médica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso do Sul. Departamento de Clínica Médica. Campo Grande, MS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Dengue NS1 ELISA | pt_BR |
| Subject | Detection | pt_BR |
| Subject | Inability | pt_BR |
| Subject | Zika infections | pt_BR |
