| Author | Jurberg, Arnon D. | |
| Author | Pascarelli, Bernardo Miguel de Oliveira | |
| Author | Machado, Marcelo Pelajo | |
| Author | Maldonado Júnior, Arnaldo | |
| Author | Mota, Ester Maria | |
| Author | Lenzi, Henrique Leonel | |
| Access date | 2012-07-30T18:42:27Z | |
| Available date | 2012-07-30T18:42:27Z | |
| Document date | 2008 | |
| Citation | JURBERG, A. D. et al. Trematode embryology: a new method for whole-egg analysis by confocal microscopy.. Development, Genes and Evolution, v. 218, p. 267-271, 2008. | pt_BR |
| ISSN | 0949-944X | |
| URI | https://www.arca.fiocruz.br/handle/icict/4250 | |
| Language | eng | pt_BR |
| Publisher | Springer Verlag | pt_BR |
| Rights | open access | |
| Title | Trematode embryology: a new method for whole-egg analysis by confocal microscopy | pt_BR |
| Type | Article | |
| DOI | DOI 10.1007/s00427-008-0209-0 | |
| Abstract | Trematode worms have the neoophoran mode of development in which several specialized vitelline cells surround the zygote. This vitelline cell mass appears just before the zygote passes through the ootype, a thickening of the oviduct, where the egg shell is formed. The great amount of vitelline material blurs the visualization of embryo development in whole egg seen by brightfield microscopy. The eggshell is difficult to cut into thin or ultrathin sections and acts as a barrier to fixation and infiltration with embedding media. The egg shell is also brightly fluorescent when analyzed by fluorescence microscopy. To overcome these technical disadvantages a simple staining protocol widely used in adult helminth morphological analysis was adapted for the study of the embryonic development of two different trematode species. The effects of potassium hydroxide as bleach and ethylene glycol as mounting medium were also evaluated. Confocal microscopy allowed virtual sectioning of whole-mounted eggs and made possible internal morphological detailed analysis of different embryonic stages. This method could contribute to the study of helminth egg embryology. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Echinostoma paraensei | pt_BR |
| Subject | Embryo | pt_BR |
| Subject | Carmine staining | pt_BR |
| Subject | Confocal microscopy | pt_BR |
| DeCS | Proteínas do Ovo | pt_BR |
| DeCS | Desenvolvimento Embrionário | pt_BR |
| DeCS | Trematódeos | pt_BR |
| DeCS | Microscopia Confocal | pt_BR |
| DeCS | Genes de Helmintos oni | pt_BR |
