| Author | Salomão, Natália | |
| Author | Rabello, Kísssila | |
| Author | Oliveira, Carlos Basílio de | |
| Author | Oliveira, Rodrigo Basílio de | |
| Author | Geraldo, Luiz | |
| Author | Lima, Flávia | |
| Author | Santos, Flávia dos | |
| Author | Nuovo, Gerard | |
| Author | Oliveira, Edson R. A. | |
| Author | Paes, Mariano | |
| Access date | 2020-08-03T18:10:24Z | |
| Available date | 2020-08-03T18:10:24Z | |
| Document date | 2020 | |
| Citation | SALOMÃO, Natália et al. Fatal Dengue Cases Reveal Brain Injury and Viral Replication in Brain-Resident Cells Associated with the Local Production of Pro-Inflammatory Mediators. Viruses, v. 12, n. 603, p. 1-16, 2020. | pt_BR |
| ISSN | 1999-4915 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42510 | |
| Language | eng | pt_BR |
| Publisher | MDPI | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Dengue | pt_BR |
| Subject in Portuguese | Casos fatais em humanos | pt_BR |
| Subject in Portuguese | Neuropatogênese | pt_BR |
| Subject in Portuguese | Inflamação | pt_BR |
| Subject in Portuguese | Sistema nervoso central | pt_BR |
| Title | Fatal Dengue Cases Reveal Brain Injury and Viral Replication in Brain-Resident Cells Associated with the Local Production of Pro-Inflammatory Mediators | pt_BR |
| Type | Article | |
| DOI | 10.3390/v12060603 | |
| Abstract | Dengue is an arboviral disease caused by dengue virus (DENV), which is transmitted to humans by Aedes aegypti mosquitoes. Infection by DENV most commonly results in a mild flu-like illness; however, the disease has been increasingly associated with neurological symptomatology. This association draws attention to further investigations on the impact of DENV infection in the host’s central nervous system. Here, we analyzed brain samples of three fatal dengue cases that occurred in 2002 during an outbreak in Rio de Janeiro, Brazil. Brain tissues of these cases were marked by histopathological alterations, such as degenerated neurons, demyelination, hemorrhage, edema, and increased numbers of astrocytes and microglial cells. Samples were also characterized by lymphocytic infiltrates mainly composed of CD8 T cells. DENV replication was evidenced in neurons, microglia and endothelial cells through immunohistochemistry and in situ hybridization techniques. Pro-inflammatory cytokines, such as TNF-α and IFN-γ were detected in microglia, while endothelial cells were marked by the expression of RANTES/CCL5. Cytoplasmic HMGB1 and the production of nitric oxide were also found in neurons and microglial cells. This work highlights the possible participation of several local pro-inflammatory mediators in the establishment of dengue neuropathogenesis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisa Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Laboratório de Ultraestrutura e Biologia de Tecidos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gafrré Guinle. Anatomia Patológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gafrré Guinle. Anatomia Patológica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Biologia Celular Glial. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Biologia Celular Glial. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Ohio State University Foundation. Ohio State University Comprehensive Cancer Center. Columbus, OH, USA / Ohio State University Foundation. Phylogeny Medical Laboratory Columbus. Columbus, OH, USA. | pt_BR |
| Affilliation | University of Illinois at Chicago. Department of Microbiology and Immunology Chicago. Chicago, IL, USA. | pt_BR |
| Subject | Dengue | pt_BR |
| Subject | Human fatal cases | pt_BR |
| Subject | Neuropathogenesis | pt_BR |
| Subject | Inflammation; | pt_BR |
| Subject | Central nervous system | pt_BR |
