| Author | Soares, Roberta Reis | |
| Author | Nakaie, Clovis Ryuichi | |
| Author | Silva, Rodrigo Nunes Rodrigues da | |
| Author | Silva, Rogério Lauria da | |
| Author | Lima Junior, Josué da Costa | |
| Author | Scopel, Kézia Katiani Gorza | |
| Access date | 2020-08-03T19:40:49Z | |
| Available date | 2020-08-03T19:40:49Z | |
| Document date | 2020 | |
| Citation | SOARES, Roberta Reis et al. Main B-cell epitopes of PvAMA-1 and PvMSP-9 are targeted by naturally acquired antibodies and epitope-specific memory cells in acute and convalescent phases of vivax malaria. Parasite Immunobiology, v. 42, p. 1-13, 2020. | pt_BR |
| ISSN | 0141-9838 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42516 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Anticorpos | pt_BR |
| Subject in Portuguese | Imunidade | pt_BR |
| Subject in Portuguese | Plasmodium vivax | pt_BR |
| Subject in Portuguese | Malária | pt_BR |
| Subject in Portuguese | Célula B de memória | pt_BR |
| Title | Main B-cell epitopes of PvAMA-1 and PvMSP-9 are targeted by naturally acquired antibodies and epitope-specific memory cells in acute and convalescent phases of vivax malaria | pt_BR |
| Type | Article | |
| DOI | 10.1111/pim.12705 | pt_BR |
| Abstract | Although antibodies are considered critical for malaria protection, little is known about the mechanisms/factors that maintain humoral immunity, especially regarding the induction and maintenance of memory B cells over time. In Brazilian endemic areas, this is the first time that the profile of antibody responses and the occurrence of antigen-specific memory B cells (MBC) against P vivax were investigated during acute malaria and up to six months after parasite clearance. For this, we selected two peptides, PvAMA-1(S290-K307) and PvMSP-9(E795-A808) , which represent the apical membrane antigen-1 and merozoite surface protein-9 of P vivax, respectively. Both peptides were previously described as containing linear B-cell epitopes. Our findings were as follows: 1-both peptides were recognized by IgG antibodies at a high frequency (between 24% and 81%) in all study groups; 2-in the absence of infection, the IgG levels remained stable throughout 6 months of follow-up; and 3-PvAMA-1(S290-K307) and PvMSP-9(E795-A808) -specific MBCs were detected in all individual groups in the absence of reinfection throughout the follow-up period, suggesting long-lived MBC. However, no positive association was observed between malaria-specific antibody levels and frequency of MBCs over time. Taken together, these results suggest that peptides can be, in the future, an alternative strategy to polypeptidic vaccine formulation. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Biofísica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia. Juiz de Fora, MG, Brasil. | pt_BR |
| Subject | Antibody | pt_BR |
| Subject | Immunity | pt_BR |
| Subject | Malaria | pt_BR |
| Subject | Memmory B Cell | pt_BR |
| Subject | Plasmodium vivax | pt_BR |
