| Author | Werneck-Barroso, E. | |
| Author | Moura, A. C. Novaes | |
| Author | Monteiro, M. Magalhães | |
| Author | Lima Júnior, O. Menezes de | |
| Author | Meirelles, M. de Nazareth Leal de | |
| Author | Henriques, M. das Graças Muller O. | |
| Access date | 2020-08-03T21:23:57Z | |
| Available date | 2020-08-03T21:23:57Z | |
| Document date | 2000 | |
| Citation | WERNECK-BARROSO, E. et al. Distinct ability to accumulate eosinophils during the inflammatory cellular response to M. bovis BCG in the mouse pleural cavity. Inflammation research, v. 49, n. 5, p. 206-213, 2000. | pt_BR |
| ISSN | 1023-3830 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42521 | |
| Language | eng | pt_BR |
| Publisher | Springer | pt_BR |
| Rights | open access | |
| Title | Distinct ability to accumulate eosinophils during the inflammatory cellular response to M. Bovis BCG in the mouse pleural cavity | pt_BR |
| Type | Article | |
| DOI | 10.1007/s000110050581 | |
| Abstract | Objective and design: The host response to Mycobacteria focuses on the development of cell-mediated immunity and granuloma formation. Here, we investigated the onset of cellular responses to mycobacteria in murine pleurisy.
Material: Distinct mouse strains previously described as Bcg susceptible or resistant were inoculated intrathoracically with different doses of live M. bovis BCG.
Methods: At various time intervals, cells harvested from the inflammatory site were identified and ultra-structurally analysed.
Results: BCG-induced pleurisy had two peaks of cellular influx at 1 and 15 days after infection. At the first half hour, macrophages were found to be heavily infected. Neutrophil arrival started after 2 h of infection and peaked at 4 h. At this time, neutrophils were found ingesting mycobacteria exclusively with a high infecting dose. BCG was potently more eosinophilotactic in Bcg susceptible mice than in the resistant ones and to other well known eosinophilia inducers: IL-5, PAF-acether or LPS.
Conclusions: Mycobacterial load and mouse susceptibility seem to determine the early granulocyte dynamics in the lesion. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil / Federal University of Rio de Janeiro. Institute of Thorax Diseases. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-Estrutura e Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos. Laboratório de Farmacologia Aplicada. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | M. bovis BCG | pt_BR |
| Subject | Neutrophil | pt_BR |
| Subject | Eosinophil | pt_BR |
| Subject | Inflammation | pt_BR |
| Embargo date | 2021-03-06 | |
