| Author | Levecke, Bruno | |
| Author | Cools, Piet | |
| Author | Albonico, Marco | |
| Author | Ame, Shaali | |
| Author | Angebault, Cécile | |
| Author | Ayana, Mio | |
| Author | Behnke, Jerzy M. | |
| Author | Bethony, Jeffrey Michael | |
| Author | Cringoli, Giuseppe | |
| Author | Dana, Daniel | |
| Author | Guillard, Bertrand | |
| Author | Hoa, Nguyen Thi Viet | |
| Author | Kang, Gagandeep | |
| Author | Kattula, Deepthi | |
| Author | Keiser, Jennifer | |
| Author | Kotze, Andrew C. | |
| Author | Matoso, Leonardo Ferreira | |
| Author | Maurelli, Maria P. | |
| Author | McCarthy, James S. | |
| Author | Mekonnen, Zeleke | |
| Author | Mirams, Greg | |
| Author | Montresor, Antonio | |
| Author | Oliveira, Rodrigo Corrêa de | |
| Author | Periago, Maria Victoria | |
| Author | Pinto, Simone Aparecida | |
| Author | Rinaldi, Laura | |
| Author | Sayasone, Somphou | |
| Author | Sumo, Laurentine | |
| Author | Tchuem-Tchuenté, Louis-Albert | |
| Author | Thach, Dang Thi Cam | |
| Author | Thomas, Eurion | |
| Author | Zeynudin, Ahmed | |
| Author | Verweij, Jaco J. | |
| Author | Vlaminck, Johnny | |
| Author | Vercruysse, Jozef | |
| Access date | 2020-08-06T11:51:16Z | |
| Available date | 2020-08-06T11:51:16Z | |
| Document date | 2020 | |
| Citation | LEVECKE, Bruno et al. Identifying thresholds to classify moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR. PLoS Negl Trop Dis, v. 14, n. 7, e0008296, 2020. | pt_BR |
| ISSN | 1935-2727 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42568 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Title | Identifying thresholds to classify moderate-to-heavy soil-transmitted helminth intensity infections for FECPAK(G2), McMaster, Mini-FLOTAC and qPCR | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pntd.0008296 | |
| Abstract | World Health Organization (WHO) has defined moderate-to-heavy intensity (M&HI) infections with soil-transmitted helminths (Ascaris lumbricoides, Trichuris trichiura and the two hookworms, Ancylostoma duodenale and Necator americanus) based on specific values of eggs per gram of stool, as measured by the Kato-Katz method. There are a variety of novel microscopy and DNA-based methods but it remains unclear whether applying current WHO thresholds on to these methods allows for a reliable classification of M&HI infections. We evaluated both WHO and method-specific thresholds for classifying the M&HI infections for novel microscopic (FECPAKG2, McMaster and Mini-FLOTAC) and DNA-based (qPCR) diagnostic methods. For this, we determined method-specific thresholds that best classified M&HI infections (defined by Kato-Katz and WHO thresholds; reference method) in two multi-country drug efficacy studies. Subsequently, we verified whether applying these method-specific thresholds improved the agreement in classifying M&HI infections compared to the reference method. When we applied the WHO thresholds, the new microscopic methods mainly misclassified M&HI as low intensity, and to a lesser extent low intensity infection as M&HI. For FECPAKG2, applying the method-specific thresholds significantly improved the agreement for Ascaris (moderate → substantial), Trichuris and hookworms (fair → moderate). For Mini-FLOTAC, a significantly improved agreement was observed for hookworms only (fair → moderate). For the other STHs, the agreement was almost perfect and remained unchanged. For McMaster, the method-specific thresholds revealed a fair to a substantial agreement but did not significantly improve the agreement. For qPCR, the method-specific thresholds based on genome equivalents per ml of DNA moderately agreed with the reference method for hookworms and Trichuris infections. For Ascaris, there was a substantial agreement. We defined method-specific thresholds that improved the classification of M&HI infections. Validation studies are required before they can be recommended for general use in assessing M&HI infections in programmatic settings. | pt_BR |
| Affilliation | Department of Virology. Parasitology and Immunology. Ghent University. Merelbeke, Belgium | pt_BR |
| Affilliation | Department of Virology. Parasitology and Immunology. Ghent University. Merelbeke, Belgium | pt_BR |
| Affilliation | Center for Tropical Diseases. Sacro Cuore Don Calabria Hospital. Negrar, Italy/Department of Life Sciences and Systems Biology. University of Turin. Turin, Italy | pt_BR |
| Affilliation | Public Health Laboratory-Ivo de Carneri. Chake Chake, United Republic of Tanzania | pt_BR |
| Affilliation | Clinical Laboratory. Institut Pasteur. Phnom Penh, Cambodia | pt_BR |
| Affilliation | Jimma University Institute of Health. Jimma University. Jimma, Ethiopia | pt_BR |
| Affilliation | School of Life Sciences. University of Nottingham. Nottingham, United Kingdom | pt_BR |
| Affilliation | Microbiology, Immunology, and Tropical Medicine. George Washington University Medical Center. Washington D.C., United States of America | pt_BR |
| Affilliation | Department of Veterinary Medicine and Animal Production. University of Naples Federico II. Naples, Italy | pt_BR |
| Affilliation | Jimma University Institute of Health. Jimma University. Jimma, Ethiopia | pt_BR |
| Affilliation | Clinical Laboratory. Institut Pasteur. Phnom Penh, Cambodia | pt_BR |
| Affilliation | Department of Parasitology. National Institute of Malariology, Parasitology and Entomology. Ha Noi, Vietnam | pt_BR |
| Affilliation | Department of Gastrointestinal Sciences. Christian Medical College. Vellore, India | pt_BR |
| Affilliation | Department of Gastrointestinal Sciences. Christian Medical College. Vellore, India | pt_BR |
| Affilliation | Department of Medical Parasitology and Infection Biology. Swiss Tropical and Public Health Institute. Basel, Switzerland/University of Basel. Basel, Switzerland | pt_BR |
| Affilliation | Division of Agriculture and Food. Commonwealth Scientific and Industrial Research Organization. St. Lucia, Australia | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Molecular e Celular. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Department of Veterinary Medicine and Animal Production. University of Naples Federico II. Naples, Italy | pt_BR |
| Affilliation | QIMR Berghofer Medical Research Institute. University of Queensland. Brisbane, Australia | pt_BR |
| Affilliation | Jimma University Institute of Health. Jimma University. Jimma, Ethiopia | pt_BR |
| Affilliation | Techion Group Ltd. Dunedin, New Zealand | pt_BR |
| Affilliation | Department of Control of Neglected Tropical Diseases. World Health Organization. Geneva, Switzerland | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Molecular e Celular. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Molecular e Celular. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Laboratório de Imunologia Molecular e Celular. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy | pt_BR |
| Affilliation | National Institute of Public Health, Ministry of Health, Vientiane, Lao People’s Democratic Republic | pt_BR |
| Affilliation | Centre for Schistosomiasis and Parasitology, University of Yaoundé I, Yaoundé, Cameroon | pt_BR |
| Affilliation | Centre for Schistosomiasis and Parasitology, University of Yaoundé, Yaoundé, Cameroon | pt_BR |
| Affilliation | Department of Parasitology, National Institute of Malariology, Parasitology and Entomology, Ha Noi, Vietnam | pt_BR |
| Affilliation | Techion Group Ltd. Aberystwyth, United Kingdom | pt_BR |
| Affilliation | Jimma University Institute of Health. Jimma University. Jimma, Ethiopia | pt_BR |
| Affilliation | Laboratory for Medical Microbiology and Immunology. Elisabeth-Tweesteden Hospital. Tilburg, The Netherlands | pt_BR |
| Affilliation | Department of Virology, Parasitology and Immunology. Ghent University. Merelbeke, Belgium | pt_BR |
| Affilliation | Department of Virology, Parasitology and Immunology. Ghent University. Merelbeke, Belgium | pt_BR |
| Subject | Helminth infections | pt_BR |
| Subject | Hookworms | pt_BR |
| Subject | Eggs | pt_BR |
| Subject | Ascaris | pt_BR |
| Subject | Trichuris | pt_BR |
| Subject | Soil-transmitted helminthiases | pt_BR |
| Subject | Diagnostic medicine | pt_BR |
| Subject | Global health | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
| xmlui.metadata.dc.subject.ods | 15 Vida terrestre | |
