| Author | Raposo, Jéssica Vasques | |
| Author | Sarmento, Dmitry José de Santana | |
| Author | Pinto, Rafaela Barbosa da Silva | |
| Author | Lopes, Amanda Oliveira | |
| Author | Gallottini, Marina | |
| Author | Tozetto-Mendoza, Tânia Regina | |
| Author | Silva, Paulo Henrique Braz | |
| Author | Paula, Salete de | |
| Access date | 2020-08-11T14:30:11Z | |
| Available date | 2020-08-11T14:30:11Z | |
| Document date | 2020 | |
| Citation | RAPOSO, Jéssica Vasques et al. Longitudinal study on oral shedding of human betaherpesviruses 6 and 7 in renal transplant recipients reveals active replication. Journal of Oral Microbiology, v. 12, p. 1-8, 2020. | pt_BR |
| ISSN | 2000-2297 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42657 | |
| Language | eng | pt_BR |
| Publisher | Taylor & Francis | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Estudo longitudinal | pt_BR |
| Subject in Portuguese | Betaherpesvírus humanos 6 | pt_BR |
| Subject in Portuguese | Betaherpesvírus humanos 7 | pt_BR |
| Subject in Portuguese | Receptores de transplante renal | pt_BR |
| Title | Longitudinal study on oral shedding of human betaherpesviruses 6 and 7 in renal transplant recipients reveals active replication | pt_BR |
| Type | Article | |
| DOI | 10.1080/20002297.2020.1785801 | |
| Abstract | Backgroung: Roseolovirus latency and persistence in salivary glands that are frequently reactivated after renal transplantation to cause infection have been reported. However, limited information is available on the persistence and excretion of HHV-6 and HHV-7 during and after transplant. Methods: 32 renal transplant recipients were followed up before (T1) and after transplant (T2 and T3) and viral replication (via assessment of mRNA) in oral fluid samples investigated. Roseolovirus DNA was detected and quantified via multiplex qPCR. For evaluation of mRNA replication, positive samples were subjected to nested RT-PCR. Results: Viral replication of HHV-7 was significantly increased during T3 (72.9%), compared to the pre-transplant period T1 (25%; McNemar Test, p= 0.001). Analysis of the viral replicative to quantitative ratio disclosed ahigher number of DNA copies (>106) in positive cases of replication (p < 0.001). Astrong positive correlation (Spearman correlation coefficient = 0.781; p< 0.001) was evident between viral quantities of Roseoloviruses. Conclusion: Our findings consistently suggest that the salivary gland is an important site of active and persistent infection by roseoloviruses. In view of the increasing problem of Roseoloviruses, pre- and post-transplantation, viral surveillance and monitoring of active replication are pivotal steps for effective screening and treatment of renal transplant patients. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Odontologia. Departamento de Estomatologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Odontologia. Departamento de Estomatologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical de São Paulo. Laboratório de Vigilância. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | niversidade de São Paulo. Faculdade de Odontologia. Departamento de Estomatologia. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Instituto de Medicina Tropical de São Paulo. Laboratório de Vigilância. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Longitudinal study | pt_BR |
| Subject | Human betaherpesviruses 6 | pt_BR |
| Subject | Human betaherpesviruses 7 | pt_BR |
| Subject | Renal transplant recipients | pt_BR |
