| Author | Queiroz, Gerson de A. | |
| Author | Silva, Raimon R. da | |
| Author | Pires, Anaque de O. | |
| Author | Costa, Ryan dos S. | |
| Author | Nevesc, Neuza Maria Alcântara | |
| Author | Silva, Thiago M. da | |
| Author | Barreto, Mauricio Lima | |
| Author | Oliveira, Sergio C. | |
| Author | Figueirêdo, Camila A. | |
| Access date | 2020-08-24T14:02:32Z | |
| Available date | 2020-08-24T14:02:32Z | |
| Document date | 2020 | |
| Citation | QUEIROZ, Gerson de A. et al. New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian population. Cytokine, 2020. | pt_BR |
| ISSN | 1043-4666 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/42907 | |
| Sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brasil (CAPES) - Finance Code 001, National Research Council, Brazil
(CNPq) and Foundation for Research Support of the State of Bahia
(FAPESB). This study was also funded by Wellcome Trust, United
Kingdom (Grant no. 072405/Z/03/Z). | pt_BR |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Asma | pt_BR |
| Subject in Portuguese | Alergia | pt_BR |
| Subject in Portuguese | Inflammassoma | pt_BR |
| Subject in Portuguese | Variantes | pt_BR |
| Subject in Portuguese | Proteína NLRP3 | pt_BR |
| Subject in Portuguese | CASP1 | pt_BR |
| Title | New variants in NLRP3 inflammasome genes increase risk for asthma and Blomia tropicalis-induced allergy in a Brazilian population | pt_BR |
| Type | Article | pt_BR |
| Abstract | Atopic asthma is a chronic lung disease of lower airways caused mainly due to action of T-helper (Th) 2 type
cytokines, eosinophilic inflammation, mucus hypersecretion and airway remodelling. Interleukin (IL)-33 increases
type 2 immunity polarization in airway playing critical role in eosinophilic asthma. On the other hand,
NLRP3 inflammasome activation results in the release of caspase-1 (Casp-1) which, in its turn, promotes IL-33
inactivation. Recent studies have shown associations between NLRP3 variants and inflammatory diseases.
However, no study with genes in NLRP3 inflammassome route has been conducted so far with asthma and atopy
in any population to date. Blood samples were collected from 1246 asthmatic and non-asthmatic children.
Associations were tested for single nucleotide polymorphism (SNP)s in NLRP3 and CASP1 with asthma and
markers of atopy and in cultures stimulated with Blomia tropicalis (Bt) mite crude extract. The T allele of
rs4925648 (NLRP3) was associated with increased asthma risk (OR 1.50, P = 0.005). In addition, the T allele of
rs12130711 polymorphism, whithin the same gene, acted as a protector factor for asthma (OR 0.78, P = 0.038).
On the other hand, the C allele of rs4378247 NLRP3 variant was associated with lower levels of IL-13 production
when peripheral blood cells were stimulated with Bt (OR 0.39, P = 4E-04). In addition, the greater the number
of risk alleles in IL33/NLRP3/CASP1 route the greater was the risk for asthma. The T allele of rs7925706 CASP1
variant was also associated with increased risk for asthma (OR 1.47, P = 0.008). In addition, this same allele
increased the eosinophil counts in blood (mm3) in asthmatic individuals compared with non-asthmatic
(P=0.0004). These results suggest that NLRP3 and CASP1 polymorphisms may be associated with susceptibility
for asthma and markers of atopy in our population. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Integração de Dados e Conhecimentos para Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Integração de Dados e Conhecimentos para Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Integração de Dados e Conhecimentos para Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Integração de Dados e Conhecimentos para Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Integração de Dados e Conhecimentos para Saúde. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Sudoeste da Bahia. Departamento de Ciências Biológicas. Jequié, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Integração de Dados e Conhecimentos para Saúde. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Saude Coletiva. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil. | pt_BR |
| Subject | Asthma | pt_BR |
| Subject | Allergy | pt_BR |
| Subject | Inflammasome | pt_BR |
| Subject | Variants | pt_BR |
| Subject | NLRP3 | pt_BR |
| Subject | CASP1 | pt_BR |
