| Author | Santos, João Conrado Khouri dos | |
| Author | Silva Filho, João Luiz | |
| Author | Judice, Carla C | |
| Author | Kayano, Ana Carolina Andrade Vitor | |
| Author | Aliberti, Júlio | |
| Author | Cunha, Antonio Ricardo Khouri | |
| Author | Lima, Diógenes S. de | |
| Author | Nakaya, Helder | |
| Author | Lacerda, Marcus Vinicius Guimarães | |
| Author | Paula, Erich Vinicius de | |
| Author | Lopes, Stefanie Costa Pinto | |
| Author | Costa, Fabio Trindade Maranhão | |
| Access date | 2020-08-31T14:33:54Z | |
| Available date | 2020-08-31T14:33:54Z | |
| Document date | 2020 | |
| Citation | SANTOS, João Conrado Khouri dos et al. Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria. LoS Neglected Tropical Diseases, 2020. | |
| URI | https://www.arca.fiocruz.br/handle/icict/43078 | |
| Sponsorship | FAPESP grants #2012/16525-2 and #2017/18611-7. JCKS receives a scholarship from CAPES. JLSF receives a scholarship from FAPESP #2916/12855-9. HN, MVGL, EVP and FTMC are CNPq research fellows. | |
| Language | eng | en_US |
| Publisher | Public Library of Science | |
| Rights | open access | |
| Subject in Portuguese | Trombocitopenia | pt_BR |
| Subject in Portuguese | Trombopoiese | pt_BR |
| Subject in Portuguese | Ativação plaquetária | pt_BR |
| Subject in Portuguese | CD40L | pt_BR |
| Subject in Portuguese | Ativação de células endoteliais | pt_BR |
| Title | Platelet disturbances correlate with endothelial cell activation in uncomplicated Plasmodium vivax malaria | en_US |
| Type | Preprint | |
| Abstract | Platelets drive endothelial cell activation in many diseases. However, if this occurs in Plasmodium vivax malaria is unclear. As platelets have been reported to be activated and to play a role in inflammatory response during malaria, we hypothesized that this would correlate with endothelial alterations during acute illness. Methods. We performed platelet flow cytometry of PAC-1 and P-selectin. We measured Platelet markers (CXCL4, CD40L, P-selectin, Thrombopoietin, IL-11) and endothelial markers (ICAM-1, von Willebrand Factor and E-selectin) in plasma with a multiplex-based assay. The values of each mediator were used to generate heatmaps, K-means clustering and Principal Component analysis. In addition, we determined pair-wise Pearson’s correlation coefficients to generate correlation networks. Results. Platelet counts were reduced, and mean platelet volume increased in malaria patients. The activation of circulating platelets in flow cytometry did not differ between patients and controls. CD40L levels (Median [IQ]: 517 [406-651] vs. 1029 [732-1267] pg/mL, P=0.0001) were significantly higher in patients, while P-selectin (Median [IQ]: 17.0 [15.4-20.6] vs. 22.2 [17.6-25.7] ng/mL, P=0.0621) and CXCL4 showed a nonsignificant trend towards higher levels in patients. The network correlation approach demonstrated the correlation between markers of platelet and endothelial activation, and the heatmaps revealed a distinct pattern of activation in two subsets of P. vivax patients when compared to controls. Conclusion. platelet activation occurs in uncomplicated vivax malaria and this correlates with higher endothelial cell activation, especially in a subset of patients. | en_US |
| Affilliation | Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil / Universidade Estadual de Campinas Faculdade de Ciências Médicas.Campinas, SP, Brasil. | |
| Affilliation | Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil. | |
| Affilliation | Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil. | |
| Affilliation | Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil. | |
| Affilliation | National Institute of Allergy and Infectious Diseases. Maryland, USA. | |
| Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil. | |
| Affilliation | University of Sao Paulo. School of Pharmaceutical Sciences. Department of Clinical and Toxicological Analyses. São Paulo, SP, Brazil.. | |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil.. | |
| Affilliation | Universidade Estadual de Campinas. Centro de Hematologia e Hemoterapia. Hemocentro. Campinas, SP, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leônidas & Maria Deane. Manaus, AM, Brasil.. | |
| Affilliation | Universidade Estadual de Campinas. Instituto de Biologia. Laboratório de Doenças Tropicais Prof. Luiz Jacintho da Silva. Campinas, SP, Brasil. | |
| Subject | Thrombocytopenia | en_US |
| Subject | Thrombopoiesis | en_US |
| Subject | Platelet activation | en_US |
| Subject | CD40L | en_US |
| Subject | P-selectin | en_US |
| Subject | Endothelial cell activation | en_US |
