| Author | Bordin, Dayanne M. | |
| Author | Latgé, Samara G. C. | |
| Author | Pyke, Graham | |
| Author | Kalman, John | |
| Author | Doble, Philip | |
| Author | Genta, Fernando A. | |
| Author | Blanes, Lucas | |
| Access date | 2020-09-03T17:49:22Z | |
| Available date | 2020-09-03T17:49:22Z | |
| Document date | 2020 | |
| Citation | BORDIN, Dayanne M. et al. A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays. Journal of the Brazilian Chemical Society, v. 31, n. 10, p. 2129-2134, 2020. | pt_BR |
| ISSN | 0103-5053 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/43169 | |
| Language | eng | pt_BR |
| Publisher | Universidade de Campinas | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Eletroforese capilar | pt_BR |
| Subject in Portuguese | Néctar | pt_BR |
| Subject in Portuguese | Sacarose | pt_BR |
| Subject in Portuguese | Glicose | pt_BR |
| Subject in Portuguese | Frutose | pt_BR |
| Title | A Simple Approach to Analyze Sugar Nectar Composition in Flowers Using Capillary Electrophoresis and Enzymatic Assays | pt_BR |
| Type | Article | |
| DOI | 10.21577/0103-5053.20200114 | |
| Abstract | Despite the obvious importance of nectar, the composition of this solution remains surprisingly
understudied to most of the flowers. Here we describe a simple, low-cost, and reliable methodology
to analyze the three main sugars present in 210 nectar samples of Christmas Bellflowers using
capillary electrophoresis (CE) and enzymatic assays. CE separation (-16 kV) was made
using an electrolyte containing 36 mM NaOH pH 12, 15 mM sorbic acid, 0.5 mM hexadecyl
trimethylammonium bromide (CTAB), and indirect UV detection. 72.9% of analyzed nectar
samples were sucrose dominant; 13.3% sucrose rich only and 13.8% were fructose rich. The
concentrations of fructose and glucose present in most of the samples were very similar, while
the amount of sucrose varied considerably. The average amounts of fructose, glucose, sucrose
and total sugar were 0.22, 0.21, 0.34 and 0.8 M, respectively. The nectar sample shows a pH
average of 5. α-Glucosidase activity was observed in 65% of the samples with an average activity
of 0.013 µU µL-1 providing a possible explanation for the similar concentrations of fructose and
glucose in the samples. Under our knowledge, this is the first article that shows the analysis of
sugars in nectar flowers using CE. | pt_BR |
| Affilliation | University of Technology Sydney. School of Mathematical and Physical Sciences. New South Wales, Australia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Macquarie University. Department of Biological Sciences. New South Wales, Australia. | pt_BR |
| Affilliation | University of Technology Sydney. School of Mathematical and Physical Sciences. New South Wales, Australia. | pt_BR |
| Affilliation | University of Technology Sydney. School of Mathematical and Physical Sciences. New South Wales, Australia. | pt_BR |
| Affilliation | Macquarie University. Department of Biological Sciences. New South Wales, Australia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | pt_BR |
| Subject | Capillary electrophoresis | pt_BR |
| Subject | Nectar | pt_BR |
| Subject | Sucrose | pt_BR |
| Subject | Glucose | pt_BR |
| Subject | Fructose | pt_BR |
| e-ISSN | 1678-4790 | |
