| Author | Petrilli, Jéssica D. | |
| Author | Müller, Igor | |
| Author | Araújo, Luana E | |
| Author | Cardoso, Thiago M. | |
| Author | Carvalho, Lucas Pedreira | |
| Author | Barros, Bruna C. | |
| Author | Teixeira, Maurício | |
| Author | Arruda, Sérgio Marcos | |
| Author | Riley, Lee W. | |
| Author | Queiroz, Adriano | |
| Access date | 2020-09-15T11:40:59Z | |
| Available date | 2020-09-15T11:40:59Z | |
| Document date | 2020 | |
| Citation | PETRILLI, Jéssica D. et al. Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon. Frontiers in Immunology, p. 1-10, Aug. 2020. | pt_BR |
| ISSN | 1664-3224 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/43365 | |
| Sponsorship | NIH FIC R25TW009338
Global Health Equity Scholarsby and in part by the
Research Program for SUS-PPSUS/BA [SUS 0027/2018].
JP was a recipient of a Ph.D. fellowship from Oswaldo
Cruz Foundation/FIOCRUZ and MT was a recipient of
a CAPES Ph.D. scholarship. AQ was a recipient of the
GHES fellowship. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Frontiers Media | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Mycobacterium tuberculosis | pt_BR |
| Subject in Portuguese | Lipídios | pt_BR |
| Subject in Portuguese | Micobacterias | pt_BR |
| Subject in Portuguese | Lipídios da membrana celular | pt_BR |
| Subject in Portuguese | inflamação | pt_BR |
| Title | Differential Host Pro-Inflammatory Response to Mycobacterial Cell Wall Lipids Regulated by the Mce1 Operon | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.3389/fimmu.2020.01848 | pt_BR |
| Abstract | The cell wall of wild-type (WT) Mycobacterium tuberculosis (Mtb), an etiologic agent of tuberculosis (TB) and a Mtb strain disrupted in a 13-gene operon mce1 (1mce1) varies by more than 400 lipid species. Here, we examined Mtb lipid-induced response in murine macrophage, as well as in human T-cell subpopulations in order to gain an insight into how changes in cell wall lipid composition may modulate host immune response. Relative to WT Mtb cell wall lipids, the non-polar lipid extracts from 1mce1 enhanced the mRNA expression of lipid-sense nuclear receptors TR4 and PPAR-g and dampened the macrophage expression of genes encoding TNF-a, IL-6, and IL-1b. Relative to untreated control, WT lipid-pre-stimulatedmacrophages fromhealthy individuals induced a higher level of CD4−CD8− double negative T-cells (DN T-cells) producing TNF-a. Conversely, compared to WT, stimulation with 1mce1 lipids induced higher mean fluorescence intensity (MFI) in IL-10-producing DN T cells. Mononuclear cells from TB patients stimulated with WT Mtb lipids induced an increased production of TNF-a by CD8+ lymphocytes. Taken together, these observations suggest that changes in mce1 operon expression during a course of infection may serve as a strategy by Mtb to evade the host pro-inflammatory responses. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Pesquisa Clínica. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Pesquisa Clínica. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Pesquisa Clínica. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratorio Avançado de Saúde Pública. Salvador, BA, Brasil. | pt_BR |
| Affilliation | University of California. School of Public Health. Division of Infectious Diseases and Vaccinology. Berkeley, CA, United States. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil. | pt_BR |
| Subject | Mycobacterium tuberculosis | pt_BR |
| Subject | Lipid-induced responses | pt_BR |
| Subject | Mce1 operon | pt_BR |
| Subject | Mycobacterial cell wall lipid | pt_BR |
| Subject | Inflammation | pt_BR |
