| Author | Takeuchi, A. Mayumi | |
| Author | Traub-Cseko, Yara M. | |
| Access date | 2020-09-18T18:35:11Z | |
| Available date | 2020-09-18T18:35:11Z | |
| Document date | 1991 | |
| Citation | TAKEUCHI, A. Mayumi; TRAUB-CSEKO, Yara M. Development of nuclear DNA probes for the typing of Trypanososma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 3, p. 307-315, July/Sept. 1991. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/43457 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Sondagens aleatórias | pt_BR |
| Subject in Portuguese | Taxonomia numérica | pt_BR |
| Subject in Portuguese | Distensões | pt_BR |
| Title | Development of nuclear DNA probes for the typing of Trypanososma cruzi | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02761991000300005 | |
| Abstract | We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Random probes | pt_BR |
| Subject | Typing | pt_BR |
| Subject | Strains | pt_BR |
| Subject | Numerical taxonomy | pt_BR |
| DeCS | Distenções | pt_BR |
| e-ISSN | 1678-8060 | |
