Author | Lima, Leila de Mendonça | |
Author | Traub-Cseko, Yara M. | |
Access date | 2020-09-18T19:37:01Z | |
Available date | 2020-09-18T19:37:01Z | |
Document date | 1991 | |
Citation | LIMA, Leila de Mendonça; TRAUB-CSEKO, Yara M. A new repetitive DNA sequence from Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, n. 4, p. 475, Oct./Dec. 1991. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/43464 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Sequência de DNA | pt_BR |
Title | A new repetitive DNA sequence from Trypanosoma cruzi | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02761991000400020 | |
Abstract | Tandemly repeated DNA sequences are found in the genome of higher eukaryotes, and have also been demonstrated in Trypanosoma cruzi. Repeated DNA sequences are potentially useful for the diagnostic detection of T. cruzi (A. Gonzales et al., 1984, Proc. Natl. Acad. Sci. USA, 81: 3356-3360). We have isoleted two clones from a genomic library of T. cruzi (Y strain) that contain, in one clone a family of at least seven copies of a repetitive sequence of approximately 600 base pairs, and in the other an independent copy of the same sequence. One copy of the repetition (HSP) and the independent clone (HCR) were sequenced by the Sanger procedure (Fig.). This sequence hybridized to four strains of T. cruzi tested and did not hybridize to eleven species of trypanosotids from five different Genera, being a good candidate for diagnostic assays. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | DNA sequence | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
e-ISSN | 1678-8060 | |