| Author | Pedrosa, Carolina da Silva Gouveia | |
| Author | Silva, Livia Goto | |
| Author | Gomes, Ismael C. | |
| Author | Souza, Leticia Rocha Quintino | |
| Author | Vitória, Gabriela | |
| Author | Ornelas, Isis M. | |
| Author | Karmirian, Karina | |
| Author | Mendes, Mayara A. | |
| Author | Salerno, José Alexandre | |
| Author | Puig-Pijuan, Teresa | |
| Author | Verissimo, Carla | |
| Author | Temerozo, Jairo Ramos | |
| Author | Furtado, Daniel R. | |
| Author | Borges, Helena L. | |
| Author | Souza, Thiago Moreno L. | |
| Author | Guimarães, Marília Zaluar P. | |
| Author | Rehen, Stevens | |
| Access date | 2020-09-23T16:09:59Z | |
| Available date | 2020-09-23T16:09:59Z | |
| Document date | 2020 | |
| Citation | PEDROSA, Gabriela da S. G. et al. Non-permissive SARS-CoV-2 infection of neural cells in the developing human brain and neurospheres. bioRxiv, p. 1-22, Sept. 2020. | |
| URI | https://www.arca.fiocruz.br/handle/icict/43570 | |
| Language | eng | en_US |
| Publisher | bioRxiv | |
| Rights | open access | |
| Subject in Portuguese | SARS-CoV-2 | pt_BR |
| Subject in Portuguese | Infecção não permissiva | pt_BR |
| Subject in Portuguese | Células neurais | pt_BR |
| Subject in Portuguese | Cérebro humano | pt_BR |
| Subject in Portuguese | Neuroesferas | pt_BR |
| Subject in Portuguese | COVID-19 | pt_BR |
| Title | Non-permissive SARS-CoV-2 infection of neural cells in the developing human brain and neurospheres | en_US |
| Type | Preprint | |
| DOI | 10.1101/2020.09.11.293951 | |
| Abstract | Coronavirus disease 2019 (COVID-19) was initially described as a viral infection of the respiratory tract. It is now known, however, that many other biological systems are affected, including the central nervous system (CNS). Neurological manifestations such as stroke, encephalitis, and psychiatric conditions have been reported in COVID-19 patients, but its neurotropic potential is still debated. Here, we investigate the presence of SARS-CoV-2 in the brain from an infant patient deceased from COVID-19. The susceptibility to virus infection was compatible with the expression levels of viral receptor ACE2, which is increased in the ChP in comparison to other brain areas. To better comprehend the dynamics of the viral infection in neural cells, we exposed human neurospheres to SARS-CoV-2. Similarly to the human tissue, we found viral RNA in neurospheres, although viral particles in the culture supernatant were not infective. Based on our observations in vivo and in vitro, we hypothesize that SARS-CoV-2 does not generate productive infection in developing neural cells and that infection of ChP weakens the blood-cerebrospinal fluid barrier allowing viruses, immune cells, and cytokines to access the CNS, causing neural damage in the young brain. | en_US |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de BiofísicaCarlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças de Populações Negligenciadas. Rio de janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | |
| Affilliation | D’Or Institute for Research and Education. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil. | |
| Subject | SARS-CoV-2 | en_US |
| Subject | Non-permissive infection | en_US |
| Subject | Neural cells | en_US |
| Subject | Human brain | en_US |
| Subject | Neurospheres | en_US |
| Subject | COVID-19 | en_US |
