Author | Miranda, Vitor Hugo Simões | |
Author | Gomes, Talita Rocha | |
Author | Eller, Dirli Emerick | |
Author | Ferraz, Lorena de Cássia Neres | |
Author | Chaves, Ana Thereza | |
Author | Bicalho, Kelly Alves | |
Author | Silva, Carlos Eduardo Calzavara | |
Author | Birbrair, Alexander | |
Author | Xavier, Marcelo Antônio Pascoal | |
Author | Goes, Alfredo Miranda de | |
Author | Oliveira, Rodrigo Corrêa de | |
Author | Alves, Érica Alessandra Rocha | |
Author | Bozzi, Adriana | |
Access date | 2020-09-28T13:29:07Z | |
Available date | 2020-09-28T13:29:07Z | |
Document date | 2020 | |
Citation | MIRANDA, Vitor Hugo Simoes et al. Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment. PLoS Negl Trop Dis, v. 14, n. 8, e0008635. https://doi.org/10.1371/journal.pntd.0008635 | pt_BR |
ISSN | 1935-2727 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/43644 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Title | Liver damage in schistosomiasis is reduced by adipose tissue-derived stem cell therapy after praziquantel treatment | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/journal.pntd.0008635 | |
Abstract | Background: In view of the potential immunosuppressive and regenerative properties of mesenchymal stem cells (MSC), we investigated whether transplantation of adipose tissue-derived stem cells (ASC) could be used to control the granulomatous reaction in the liver of mice infected with Schistosoma mansoni after Praziquantel (PZQ) treatment.
Methodology/Prinicpal findings: C57BL/6 mice infected with S. mansoni were treated with PZQ and transplanted intravenously with ASC from uninfected mice. Liver morpho-physiological and immunological analyses were performed. The combined PZQ/ASC therapy significantly reduced the volume of hepatic granulomas, as well as liver damage as measured by ALT levels. We also observed that ASC accelerated the progression of the granulomatous inflammation to the advanced/curative phase. The faster healing interfered with the expression of CD28 and CTLA-4 molecules in CD4+ T lymphocytes, and the levels of IL-10 and IL-17 cytokines, mainly in the livers of PZQ/ASC-treated mice.
Conclusions: Our results show that ASC therapy after PZQ treatment results in smaller granulomas with little tissue damage, suggesting the potential of ASC for the development of novel therapeutic approaches to minimize hepatic lesions as well as a granulomatous reaction following S. mansoni infection. Further studies using the chronic model of schistosomiasis are required to corroborate the therapeutic use of ASC for schistosomiasis. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Patologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil/ Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Instituto René Rachou, Fiocruz Minas, Belo Horizonte, Minas Gerais, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil/Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brazil | pt_BR |
Subject | Granulomas | pt_BR |
Subject | Stem cell therapy | pt_BR |
Subject | Cytokines | pt_BR |
Subject | Schistosoma mansoni | pt_BR |
Subject | Mesenchymal stem cells | pt_BR |
Subject | Schistosomiasis | pt_BR |
Subject | Cytokine therapy | pt_BR |
Subject | Eggs | pt_BR |