| Author | Pereira Neto, Tertuliano Alves | |
| Author | Pereira, Marcela Helena Gonçalves | |
| Author | Queiroz, Camila Pereira de | |
| Author | Ramos, Michele Faria | |
| Author | Oliveira, Fernanda de Fátima Souza de | |
| Author | Prado, Roberta Oliveira | |
| Author | Nascimento, Valdinete Alves do | |
| Author | Abdalla, Lígia Fernandes | |
| Author | Santos, João Hugo Abdalla | |
| Author | Martins Filho, Olindo Assis | |
| Author | Naveca, Felipe Gomes | |
| Author | Carvalho, Andrea Teixeira de | |
| Author | Santiago, Helton da Costa | |
| Access date | 2020-10-07T14:14:57Z | |
| Available date | 2020-10-07T14:14:57Z | |
| Document date | 2020 | |
| Citation | PEREIRA NETO, Tertuliano Alves et al. Multifunctional T cell response in convalescent patients two years after ZIKV infection. Journal of Leukocyte Biology, v. 108, n. 4, p. 1265-1277, 2020 | pt_BR |
| ISSN | 0741-5400 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/43850 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Multifunctional T cell response in convalescent patients two years after ZIKV infection | pt_BR |
| Type | Article | |
| DOI | 10.1002/JLB.4MA0520-708R | |
| Abstract | Zika is an important emerging infectious disease in which the role of T cells remains elusive. This study aimed to evaluate the phenotype of multifunctional T cells in individuals 2 yr after exposure to Zika virus (ZIKV). We used a library of 671 synthetic peptides covering the whole polyprotein of ZIKV in pools corresponding to each viral protein (i.e., capsid, membrane precursor or prM, envelope, NS1 [nonstructural protein], NS2A + NS2B, NS3, NS4A + NS4B, and NS5) to stimulate PBMCs from individuals previously exposed to ZIKV. We observed an increased frequency of ZIKV-specific IFN , IL-17A, TNF, and IL-10 production by T cell populations. IFN and TNF production were especially stimulated by prM, capsid, or NS1 in CD8+ T cells and by capsid or prM in CD4+ T cells. In addition, there was an increase in the frequency of IL-10+ CD8+ T cells after stimulation with prM, capsid, NS1, NS3, or NS5. Multifunctional properties were observed in ZIKV- specific T cells responding especially to prM, capsid, NS1 or, to a smaller extent, NS3 antigens. For example, we found a consistent IFN + TNF+ CD8+ T cell population in response to most virus antigens and CD4+ and CD8+ T cells that were IFN + IL-17A+ and IL-17A+IL-10+, which could also produce TNF, in response to capsid, prM, NS1, or NS3 stimulation. Interestingly, CD8+ T cells were more prone to a multifunctional phenotype than CD4+ T cells, and multifunctional T cells were more efficient at producing cytokines than single-function cells. This work provides relevant insights into the quality of ZIKV-specific T cell responses and ZIKV immunity. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Análises Clínicas e Toxicologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | immunity | pt_BR |
| Subject | multifunctional T cells | pt_BR |
| Subject | Zika | pt_BR |
| Subject | ZIKV | pt_BR |
