| Author | Sousa, Maria Auxiliadora de | |
| Author | Pereira, Mirian Claudia de Souza | |
| Author | Corte-Real, Suzana | |
| Access date | 2020-10-09T13:26:06Z | |
| Available date | 2020-10-09T13:26:06Z | |
| Document date | 1997 | |
| Citation | SOUSA, Maria Auxiliadora; PEREIRA, Mirian Claudia de Souza; CÔRTE-REAL, Suzana. Leishmania major: Parasite Interactions Suggesting Sexuality. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 761-766, Nov./Dec. 1997. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/43897 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosomatidae | pt_BR |
| Subject in Portuguese | Leishmania major | pt_BR |
| Subject in Portuguese | Sexualidade | pt_BR |
| Subject in Portuguese | Morfologia | pt_BR |
| Subject in Portuguese | Imunocytoquímica | pt_BR |
| Subject in Portuguese | Microscopia eletrônica de varredura | pt_BR |
| Title | Leishmania major: Parasite Interactions Suggesting Sexuality | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02761997000600006 | |
| Abstract | In five experiments, Leishmania (Leishmania) major (MRHO/SU/59/P-strain) grew poorly when
seeded in FYTS medium supplemented with 15% fetal calf serum, but presented several peculiar pairs of
promastigotes diametrically opposed and attached at their posterior ends (5.8-13.5%). As seen in Giemsa-stained smears, a ring-like line and/or an enlargement, generally occurred at the parasite junction. A close proximity of nuclei, which sometimes were difficult to distinguish from each other, was also
observed at this junction. Several of these pairs appeared to be composed of fused cells in which the
nuclei could be apparently fused, as shown by fluorescence microscopy to detect ß-tubulin and DNA,
and by scanning electron microscopy. Under other culture conditions these pairs were absent or occurred at very low rates (0.2-2.2%). Such pairs differ markedly from longitudinally dividing cells and
resemble those described in two other Leishmania species, as well as in Herpetomonas megaseliae and
Phytomonas davidi, suggesting steps of a putative sexual process. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura e Biologia Celular. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosomatidae | pt_BR |
| Subject | Leishmania major | pt_BR |
| Subject | Sexuality | pt_BR |
| Subject | Morphology | pt_BR |
| Subject | Immunocytochemistry | pt_BR |
| Subject | Scanning electron microscopy | pt_BR |
| e-ISSN | 1678-8060 | |
