| Author | Zingales, Bianca | |
| Author | Pereira, Maria Elizabeth S. | |
| Author | Almeida, Kátia A. | |
| Author | Umezawa, Eufrosina S. | |
| Author | Nehme, Nédia S. | |
| Author | Oliveira, Riva P. | |
| Author | Macedo, Andrea | |
| Author | Souto, Ricardo P. | |
| Access date | 2020-10-09T13:55:49Z | |
| Available date | 2020-10-09T13:55:49Z | |
| Document date | 1997 | |
| Citation | ZINGALEV, Bianca et al. Biological Parameters and Molecular Markers of Clone CL Brener - The Reference Organism of the Trypanosoma cruzi Genome Project. Memórias do Instituo Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 811-814, Nov./Dec. 1997. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/43901 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | CL Brener | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Projeto Genoma | pt_BR |
| Subject in Portuguese | Características biológicas | pt_BR |
| Subject in Portuguese | Tipagem Molecular | pt_BR |
| Title | Biological Parameters and Molecular Markers of Clone CL Brener - The Reference Organism of the Trypanosoma cruzi Genome Project | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02761997000600016 | |
| Abstract | Clone CL Brener is the reference organism used in the Trypanosoma cruzi Genome Project. Some
biological parameters of CL Brener were determined: (a) the doubling time of epimastigote forms cultured in liver infusion-tryptose (LIT) medium at 28oC is 58±13 hr; (b) differentiation of epimastigotes to
metacyclic trypomastigotes is obtained by incubation in LIT-20% Grace´s medium; (c) trypomastigotes
infect mammalian cultured cells and perform the complete intracellular cycle at 33 and 37oC; (d) blood
forms are highly infective to mice; (e) blood forms are susceptible to nifurtimox and benznidazole. The
molecular typing of CL Brener has been determined: (a) isoenzymatic profiles are characteristic of
zymodeme ZB; (b) PCR amplification of a 24Sα ribosomal RNA sequence indicates it belongs to T.
cruzi lineage 1; (c) schizodeme, randomly amplified polymorphic DNA (RAPD) and DNA fingerprinting
analyses were performed. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Química. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Sem afiliação | pt_BR |
| Subject | CL Brener | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Genome Project | pt_BR |
| Subject | Biological characteristics | pt_BR |
| Subject | Molecular typing | pt_BR |
| e-ISSN | 1678-8060 | |
