| Author | Moraes, Márcia T. B. | |
| Author | Silva, Messias da | |
| Author | Leite, José Paulo G. | |
| Author | Nascimento, Jussara P. | |
| Access date | 2020-10-12T12:42:17Z | |
| Available date | 2020-10-12T12:42:17Z | |
| Document date | 1998 | |
| Citation | MORAES, Márcia T. B. et al. Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n.2, p. 231-235, Mar./Apr. 1998. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/43929 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Análise genética e antigênica | pt_BR |
| Subject in Portuguese | Adenovirus type 3 | pt_BR |
| Title | Genetic and Antigenic Analysis of Adenovirus Type 3 Strains Showing Intermediate Behavior in Standard Seroneutralization Test | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02761998000200019 | |
| Abstract | During an epidemiological survey of acute respiratory infection in Rio de Janeiro, among 208 adenovirus isolates, we found two strains that we were not able, by a standard neutralization procedure, to
distinguish between type 3 or 7. However, DNA restriction pattern for the two strains with different
enzymes were analyzed and showed a typical Ad3h profile. Using a cross-neutralization test in which
both Ad3p and Ad7p antisera were used in different concentration against 100 TCID50 of each adenovirus standard and both isolates, we were able to confirm that the two isolates belong to serotype 3. An
hemagglutination inhibition test also corroborated the identification of both strains as adenovirus type
3. Comparing Ad3h and Ad3p genome, we observed 16 different restriction enzyme sites, three of which
were located in genomic regions encoding polypeptides involved in neutralization sites. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Adenovirus type 3 | pt_BR |
| Subject | Genetic and antigenic analysis | pt_BR |
| e-ISSN | 1678-8060 | |
