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AutorAbreu, Iliano V. Coutinho
AutorOristian, James
AutorCastro, Waldionê de
AutorWilson, Timothy R.
AutorMeneses, Claudio
AutorSoares, Rodrigo P.
AutorBorges, Valéria de Matos
AutorDescoteaux, Albert
AutorKamhawi, Shaden
AutorValenzuela, Jesus G.
Data de acesso2020-10-19T11:05:55Z
Data de disponibilização2020-10-19T11:05:55Z
Data do publicação2020
CitaçãoABREU, Iliano V. Coutinho et al. Binding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Flies. mSphere, v. 5, n. 5, p. e00594-20, 2020.pt_BR
ISSNs. dpt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/44044
FomentoIntramural Research Program of the NIH, National Institute of Allergy and Infectious Diseases. A.D. holds the Canada Research Chair on the Biology of Intracellular Parasitismpt_BR
Idiomaengpt_BR
EditorAmerican Society for Microbiologypt_BR
Direito Autoralopen accesspt_BR
Palavras-chaveLeishmaniapt_BR
Palavras-chaveParasitapt_BR
Palavras-chaveSobrevivênciapt_BR
Palavras-chaveFlebotomospt_BR
Palavras-chaveIntestinopt_BR
Palavras-chaveSalivapt_BR
Palavras-chaveLeishmania infantumpt_BR
TítuloBinding of Leishmania infantum Lipophosphoglycan to the Midgut Is Not Sufficient To Define Vector Competence in Lutzomyia longipalpis Sand Fliespt_BR
Tipo do documentoArticlept_BR
DOI10.1128/mSphere.00594-20
Resumo em InglêsThe major surface lipophosphoglycan (LPG) of Leishmania parasites is critical to vector competence in restrictive sand fly vectors in mediating Leishmania attachment to the midgut epithelium, considered essential to parasite survival and development. However, the relevance of LPG for sand flies that harbor multiple species of Leishmania remains elusive. We tested binding of Leishmania infantum wildtype (WT), LPG-defective (Δlpg1 mutants), and add-back (Δlpg1 LPG1) lines to sand fly midguts in vitro and their survival in Lutzomyia longipalpis sand flies in vivo. Le. infantum WT parasites attached to the Lu. longipalpis midgut in vitro, with late-stage parasites binding to midguts in significantly higher numbers than were seen with early-stage promastigotes. Δlpg1 mutants did not bind to Lu. longipalpis midguts, and this was rescued in the Δlpg1 LPG1 lines, indicating that midgut binding is mediated by LPG. When Lu. longipalpis sand flies were infected with the Le. infantum WT or Le. infantum Δlpg1 or Le. infantum Δlpg1 LPG1 line of the BH46 or BA262 strains, the BH46 Δlpg1 mutant, but not the BA262 Δlpg1 mutant, survived and grew to numbers similar to those seen with the WT and Δlpg1 LPG1 lines. Exposure of BH46 and BA262 Δlpg1 mutants to blood-engorged midgut extracts led to mortality of the BA262 Δlpg1 but not the BH46 Δlpg1 parasites. These findings suggest that Le. infantum LPG protects parasites on a strain-specific basis early in infection, likely against toxic components of blood digestion, but that it is not necessary to prevent Le. infantum evacuation along with the feces in the permissive vector Lu. longipalpis.pt_BR
AfiliaçãoNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
AfiliaçãoNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
AfiliaçãoNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
AfiliaçãoNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
AfiliaçãoNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
AfiliaçãoFundação Oswaldo Cruz. Instituto René rachou. Belo Horizonte, MG, Brasil.
AfiliaçãoFundação Oswaldo Cruz. Instituto Gonçalo Moniz, Salvador, BA, Brasil.
AfiliaçãoCentre Armand-Frappier Santé Biotechnologie. Laval, Quebec, Canada.
AfiliaçãoNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
AfiliaçãoNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Vector Molecular Biology Section, Laboratory of Malaria and Vector Research. Rockville, Maryland, USA.
Palavras-chave em inglêsLPGpt_BR
Palavras-chave em inglêsLeishmaniapt_BR
Palavras-chave em inglêsParasite bindingpt_BR
Palavras-chave em inglêsParasite survivalpt_BR
Palavras-chave em inglêsSand flypt_BR
Palavras-chave em inglêsSand fly midgutpt_BR
Palavras-chave em inglêsVector competencept_BR
Palavras-chave em inglêsLeishmania infantumpt_BR


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