| Author | De Carli, Silvia | |
| Author | Wolf, Jonas Michel | |
| Author | Gräf, Tiago | |
| Author | Lehmann, Fernanda K. M. | |
| Author | Fonseca, André S. K. | |
| Author | Canal, Cláudio W. | |
| Author | Lungea, Vagner R. | |
| Author | Ikuta, Nilo | |
| Access date | 2020-10-19T12:26:53Z | |
| Available date | 2020-10-19T12:26:53Z | |
| Document date | 2020 | |
| Citation | DE CARLI, Silvia et al. Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil. Avian Pathology, 2020. | pt_BR |
| ISSN | 0307-9457 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44046 | |
| Language | eng | pt_BR |
| Publisher | Taylor & Francis | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | ARV | pt_BR |
| Subject in Portuguese | Tenossinovite | pt_BR |
| Subject in Portuguese | Artrite | pt_BR |
| Subject in Portuguese | Filogenia | pt_BR |
| Subject in Portuguese | Evolução viral | pt_BR |
| Title | Genotypic characterization and molecular evolution of avian reovirus in poultry flocks from Brazil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1080/03079457.2020.1804528 | |
| Abstract | Avian reovirus (ARV) is one of the main causes of infectious arthritis/tenosynovitis and
malabsorption syndrome (MAS) in poultry. ARVs have been disseminated in Brazilian poultry
flocks in the last years. This study aimed to genotype ARVs and to evaluate the molecular
evolution of the more frequent ARV lineages detected in Brazilian poultry-producing farms. A
total of 100 poultry flocks with clinical signs of tenosynovitis/MAS, from all Brazilian poultryproducing
regions were positive for ARV by PCR. Seventeen bird tissues were submitted to
cell culture and ARV RNA detection/genotyping by two PCRs. The phylogenetic classification
was based on σC gene alignment using a dataset with other Brazilian and worldwide ARVs
sequences. ARVs were specifically detected by both PCRs from the 17 cell cultures, and σC
gene partial fragments were sequenced. All these sequences were aligned with a total of 451
ARV σC gene data available in GenBank. Phylogenetic analysis demonstrated five welldefined
clusters that were classified into lineages I, II, III, IV, and V. Three lineages could be
further divided into sub-lineages: I (I vaccine, Ia, Ib), II (IIa, IIb, IIc) and IV (IVa and IVb).
Brazilian ARVs were from four lineages/sub-lineages: Ib (48.2%), IIb (22.2%), III (3.7%) and V
(25.9%). The Bayesian analysis demonstrated that the most frequent sub-lineage Ib emerged
in the world around 1968 and it was introduced into Brazil in 2010, with increasing spread
soon after. In conclusion, four different ARV lineages are circulating in Brazilian poultry flocks,
all associated with clinical diseases. | pt_BR |
| xmlui.metadata.dc.description.abstractes | Conselho Nacional de Desenvolvimento
Científico e Tecnológico (CNPq) [311010/
2017-2], Coordenação de Aperfeiçoamento de Pessoal de
Nível Superior (CAPES) [Finance Code 001], Fundação de
Amparo à Pesquisa do Estado do Rio Grande do Sul
(FAPERGS) and Pro Reitoria de Pesquisa Universidade Federal
do Rio Grande do Sul. | pt_BR |
| Affilliation | Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil / Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Virologia. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil. | pt_BR |
| Affilliation | Simbios Biotecnologia. Cachoeirinha, RS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Laboratório de Virologia. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil / Simbios Biotecnologia. Cachoeirinha, RS, Brasil. | pt_BR |
| Affilliation | Universidade Luterana do Brasil. Laboratório de Diagnóstico Molecular. Programa de Pós-Graduação em Biologia Celular e Molecular aplicada à Saúde, Canoas, RS, Brasil / Simbios Biotecnologia. Cachoeirinha, RS, Brasil. | pt_BR |
| Subject | ARV | pt_BR |
| Subject | Tenosynovitis | pt_BR |
| Subject | Arthritis | pt_BR |
| Subject | Malabsorption syndrome | pt_BR |
| Subject | Phylogeny | pt_BR |
| Subject | Viral evolution | pt_BR |
