| Author | Schottelius, J. | |
| Author | Hünger, F. | |
| Author | Schüler, T. H. | |
| Author | Costa, S. C. Gonçalves da | |
| Access date | 2020-10-20T18:19:57Z | |
| Available date | 2020-10-20T18:19:57Z | |
| Document date | 2000 | |
| Citation | SCHOTTELIUS, J. et al. Chitinolytic Activity in Viable Spores of Encephalitozoon Species. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 95, n. 5, p. 701-705, Sept./Oct. 2000. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44091 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Microspora | pt_BR |
| Subject in Portuguese | Inibição | pt_BR |
| Subject in Portuguese | Cellulase | pt_BR |
| Subject in Portuguese | Atividade de Chitinase | pt_BR |
| Title | Chitinolytic Activity in Viable Spores of Encephalitozoon Species | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762000000500015 | |
| Abstract | By employing 4-methylumbelliferyl-beta-D-NN’,N”-triacetylchitotriose substrate in a semi quantitative assay, chitinolytic activity in viable spores of Encephalitozoon cuniculi and E. intestinalis was
detected and dependence on reaction time, spore concentration, concentration of substrate and temperature were demonstrated. It was possible to block the chitinolytic activity by chitin hydrolysate. By
incubation at 80°C for 10 min or at 55°C for 20 min the spores were loosing the chitinolytic activity.
Incubation of the spores in trypsin reduced the chitinolytic activity. Cellulase activity could not be
detected. | pt_BR |
| Affilliation | Bernhard-Nocht-Institute for Tropical Medicine. Section of Parasitology. Hamburg, Germany. | pt_BR |
| Affilliation | Bernhard-Nocht-Institute for Tropical Medicine. Medical Microbiology Section. Hamburg, Germany. | pt_BR |
| Affilliation | Bernhard-Nocht-Institute for Tropical Medicine. Laboratory Animal Facilities. Hamburg, Germany. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Laboratório de Imunomodulação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Microspora | pt_BR |
| Subject | Cellulase | pt_BR |
| Subject | Chitinase activity | pt_BR |
| Subject | Inhibition | pt_BR |
| e-ISSN | 1678-8060 | |
