Author | Toaldo, C. B. | |
Author | Steindel, M. | |
Author | Spusa, M. A. | |
Author | Tavares, C. C. | |
Access date | 2020-10-22T13:46:22Z | |
Available date | 2020-10-22T13:46:22Z | |
Document date | 2001 | |
Citation | TOALDO, C. B. et al. Molecular Karyotype and Chromosomal Localization of Genes Encoding β-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 1, p. 113-121, Jan. 2001. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44111 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma rangeli | pt_BR |
Subject in Portuguese | Cariótipo | pt_BR |
Subject in Portuguese | Eletroforese em gel de campo pulsado | pt_BR |
Subject in Portuguese | Genes domésticos | pt_BR |
Subject in Portuguese | Variabilidade genética | pt_BR |
Subject in Portuguese | Fenograma | pt_BR |
Title | Molecular Karyotype and Chromosomal Localization of Genes Encoding ß-tubulin, Cysteine Proteinase, hsp 70 and Actin in Trypanosoma rangeli | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762001000100013 | |
Abstract | The molecular karyotype of nine Trypanosoma rangeli strains was analyzed by contour-clamped
homogeneous electric field electrophoresis, followed by the chromosomal localization of β-tubulin, cysteine proteinase, 70 kDa heat shock protein (hsp 70) and actin genes. The T. rangeli strains were isolated from either insects or mammals from El Salvador, Honduras, Venezuela, Colombia, Panama and
southern Brazil. Also, T. cruzi CL-Brener clone was included for comparison. Despite the great similarity observed among strains from Brazil, the molecular karyotype of all T. rangeli strains analyzed revealed extensive chromosome polymorphism. In addition, it was possible to distinguish T. rangeli from
T. cruzi by the chromosomal DNA electrophoresis pattern. The localization of β-tubulin genes revealed
differences among T. rangeli strains and confirmed the similarity between the isolates from Brazil. Hybridization assays using probes directed to the cysteine proteinase, hsp 70 and actin genes discriminated T. rangeli from T. cruzi, proving that these genes are useful molecular markers for the differential
diagnosis between these two species. Numerical analysis based on the molecular karyotype data revealed a high degree of polymorphism among T. rangeli strains isolated from southern Brazil and strains
isolated from Central and the northern South America. The T. cruzi reference strain was not clustered
with any T. rangeli strain. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal de Santa Catarina. Departamento de Microbiologia e Parasitologia., Florianópolis, SC, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Coleção de Tripanosomatídeos. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade do Estado do Rio de Janeiro. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Trypanosoma rangeli | pt_BR |
Subject | Karyotype | pt_BR |
Subject | Pulsed field gel electrophoresis | pt_BR |
Subject | Housekeeping genes | pt_BR |
Subject | Genetic variability | pt_BR |
Subject | Phenogram | pt_BR |
e-ISSN | 1678-8060 | |