| Author | Gruenberg, Maria | |
| Author | Moniz, Clara Antunes | |
| Author | Hofmann, Natalie E. | |
| Author | Koepfli, Cristian | |
| Author | Robinson, Leanne J. | |
| Author | Nate, Elma | |
| Author | Monteiro, Wuelton Marcelo | |
| Author | Melo, Gisely Cardoso de | |
| Author | Kuehn, Andrea | |
| Author | Siqueira, André M. | |
| Author | Nguitragool, Wang | |
| Author | Bassat, Quique | |
| Author | Lacerda, Marcus | |
| Author | Sattabongkot, Jetsumon | |
| Author | Mueller, Ivo | |
| Author | Felger, Ingrid | |
| Access date | 2020-10-22T16:44:32Z | |
| Available date | 2020-10-22T16:44:32Z | |
| Document date | 2020 | |
| Citation | GRUENBERG, Maria et al. Utility of ultra-sensitive qPCR to detect Plasmodium falciparum and Plasmodium vivax infections under different transmission intensities. Malaria Journal, v. 19, n. 1, p. 1-10, Sep. 2020. | pt_BR |
| ISSN | 1475-2875 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44119 | |
| Sponsorship | This study was funded by the Swiss National Science Foundation (grants no. 310030_159580 and IZRJZ3_164182), the TransEPi Consortium/Bill and Melinda Gates Foundation (OPP1034577) and by the NIH (1 U19 AI089686-01). IM was supported by an NHMRC Senior Research Fellowship. | |
| Language | eng | pt_BR |
| Publisher | BioMed Central | pt_BR |
| Rights | open access | pt_BR |
| Title | Utility of ultra-sensitive qPCR to detect Plasmodium falciparum and Plasmodium vivax infections under different transmission intensities | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1186/s12936-020-03374-7 | |
| Abstract | Background: The use of molecular diagnostics has revealed an unexpectedly large number of asymptomatic low-density malaria infections in many malaria endemic areas. This study compared the gains in parasite prevalence obtained by the use of ultra-sensitive (us)-qPCR as compared to standard qPCR in cross-sectional surveys conducted in Thailand, Brazil and Papua New Guinea (PNG). The compared assays differed in the copy number of qPCR targets in the parasite genome. Methods: Plasmodium falciparum (Pf) and Plasmodium vivax (Pv) parasites were quantified by qPCR amplifying the low-copy Pf_ and Pv_18S rRNA genes or the multi-copy targets Pf_varATS and Pv_mtCOX1. Cross-sectional surveys at the three study sites included 2252 participants of all ages and represented different transmission intensities. Results: In the two low-transmission areas, P. falciparum positivity was 1.3% (10/773) (Thailand) and 0.8% (5/651) (Brazil) using standard Pf_18S rRNA qPCR. In these two countries, P. falciparum positivity by Pf_varATS us-qPCR increased to 1.9% (15/773) and 1.7% (11/651). In PNG, an area with moderate transmission intensity, P. falciparum positivity significantly increased from 8.6% (71/828) by standard qPCR to 12.2% (101/828) by us-qPCR. The proportions of P. falciparum infections not detected by standard qPCR were 33%, 55% and 30% in Thailand, Brazil and PNG. Plasmodium vivax was the predominating species in Thailand and Brazil, with 3.9% (30/773) and 4.9% (32/651) positivity by Pv_18S rRNA qPCR. In PNG, P. vivax positivity was similar to P. falciparum, at 8.0% (66/828). Use of Pv_mtCOX1 us-qPCR led to a significant increase in positivity to 5.1% (39/773), 6.4% (42/651) and 11.5% (95/828) in Thailand, Brazil, and PNG. The proportions of P. vivax infections missed by standard qPCR were similar at all three sites, with 23%, 24% and 31% in Thailand, Brazil and PNG. Conclusion: The proportional gains in the detection of P. falciparum and P. vivax infections by ultra-sensitive diagnostic assays were substantial at all three study sites. Thus, us-qPCR yields more precise prevalence estimates for both P. falciparum and P. vivax at all studied levels of endemicity and represents a significant diagnostic improvement. Improving sensitivity in P. vivax surveillance by us-qPCR is of particular benefit, because the additionally detected P. vivax infections signal the potential presence of hypnozoites and subsequent risk of relapse and further transmission. | pt_BR |
| Affilliation | Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland. | pt_BR |
| Affilliation | Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland. | pt_BR |
| Affilliation | Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland. | pt_BR |
| Affilliation | Walter and Eliza Hall Institute of Medical Research. Melbourne, Australia / University of Notre Dame. Eck Institute for Global Health. Notre Dame, IN, USA. | pt_BR |
| Affilliation | Papua New Guinea Institute of Medical Research. Madang, Papua New Guinea / Burnet Institute. Melbourne, Australia. | pt_BR |
| Affilliation | Papua New Guinea Institute of Medical Research. Madang, Papua New Guinea. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil / Universitat de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Spain. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil / Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Mahidol University. Faculty of Tropical Medicine. Department of Molecular Tropical Medicine & Genetics. Bangkok, Thailand. | pt_BR |
| Affilliation | Universitat de Barcelona. Hospital Clínic. ISGlobal. Barcelona, Spain. | pt_BR |
| Affilliation | Fundação de Medicina Tropical Dr. Heitor Vieira Dourado (FMT-HVD). Manaus, AM, Brasil / Universidade do Estado do Amazonas. Manaus, AM, Brasil. | pt_BR |
| Affilliation | Mahidol University. Faculty of Tropical Medicine. Mahidol Vivax Research Unit Bangkok, Thailand. | pt_BR |
| Affilliation | Walter and Eliza Hall Institute of Medical Research. Melbourne, Australia / University of Melbourne. Department of Medical Biology. Melbourne, Australia / Institut Pasteur. Malaria Parasite & Hosts Unit. Paris, France. | pt_BR |
| Affilliation | Swiss Tropical and Public Health Institute. Basel, Switzerland / University of Basel. Basel, Switzerland. | pt_BR |
| Subject | Low-density | pt_BR |
| Subject | Molecular diagnostics | pt_BR |
| Subject | Ultra-sensitive | pt_BR |
| Subject | mtCOX1 | pt_BR |
| Subject | qPCR | pt_BR |
| Subject | varATS | pt_BR |
| e-ISSN | 1475-2875 | |
