| Author | Thaumaturgo, Nilton | |
| Author | Vilar, Mônica Magno | |
| Author | Diogo, Catia Maria | |
| Author | Edelenyi, Ricardo | |
| Author | Tendler, Miriam | |
| Access date | 2020-10-24T17:58:16Z | |
| Available date | 2020-10-24T17:58:16Z | |
| Document date | 2001 | |
| Citation | THAUMATURGO, Nilton et al. Preliminary Analysis of Sm14 in Distinct Fractions of Schistosoma mansoni Adult Worm Extract. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, Suppl., p. 79-83, 2001. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44169 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Schistosoma mansoni | pt_BR |
| Subject in Portuguese | Antígenos excretórios | pt_BR |
| Subject in Portuguese | FABP | pt_BR |
| Subject in Portuguese | Vacina | pt_BR |
| Title | Preliminary Analysis of Sm14 in Distinct Fractions of Schistosoma mansoni Adult Worm Extract | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762001000900011 | |
| Abstract | In previous studies it was shown that the recombinant molecule, r-Sm14, induces high levels of
protection against Schistosoma mansoni infection in two outbred animal models and immune
crossprotection against infection by Fasciola hepatica in Swiss outbred mice. r-Sm14 was derived from
a living worm extract, called SE, and is being developed as the molecular basis of an anti-helminth
bivalent vaccine against the two parasites, for medical and veterinary application. Present data refer
to SDS-PAGE and Western Blotting analysis of four different preparations of S. mansoni adult worms
focusing Sm14 identification. The extracts correspond to the initial fraction of the SE extraction process, containing products released by living worms (SEi); SE2, reextraction of adult worms in PBS; and
SE of separated male and female adult worms. In all extracts it was possible to detect the component of
14 kDa, that was recognized by specific anti-rSm14 antibody raised in rabbits. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Excretory/secretory antigens | pt_BR |
| Subject | Vaccine | pt_BR |
| Subject | FABP | pt_BR |
| Subject | Sm14 | pt_BR |
| e-ISSN | 1678-8060 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
