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| Autor | Silva Jr, F. P. | |
| Autor | Veyl, F. Z. | |
| Autor | Clos, J. | |
| Autor | De Simone, Salvatore Giovanni | |
| Fecha de acceso | 2020-10-25T19:45:24Z | |
| Fecha de disponibilización | 2020-10-25T19:45:24Z | |
| Fecha de publicación | 2002 | |
| Referencia | SILVA JR, F. P. et al. Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 3, p. 335-341, Apr. 2002. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44181 | |
| Idioma | eng | pt_BR |
| Editor | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Derechos de autor | open access | |
| Palabras clave en Portugués | Modelagem molecular | pt_BR |
| Palabras clave en Portugués | Proteína de ligação poli-A | pt_BR |
| Palabras clave en Portugués | Leishmania amazonensis | pt_BR |
| Palabras clave en Portugués | Bioinformatics | pt_BR |
| Título | Molecular Modeling Approaches for Determining Gene Function: Application to a Putative Poly-A Binding Protein from Leishmania amazonensis (LaPABP) | pt_BR |
| Tipo del documento | Article | |
| DOI | 10.1590/S0074-02762002000300010 | |
| Resumen en Inglés | he great expansion in the number of genome sequencing projects has revealed the importance of computational methods to speed up the characterization of unknown genes. These studies have been improved by the use of three dimensional information from the predicted proteins generated by molecular modeling techniques. In this work, we disclose the structure-function relationship of a gene product from Leishmania amazonensis by applying molecular modeling and bioinformatics techniques. The analyzed sequence encodes a 159 aminoacids polypeptide (estimated 18 kDa) and was denoted LaPABP for its high homology with poly-A binding proteins from trypanosomatids. The domain structure, clustering analysis and a three dimensional model of LaPABP, basically obtained by homology modeling on the structure of the human poly-A binding protein, are described. Based on the analysis of the electrostatic potential mapped on the model's surface and conservation of intramolecular contacts responsible for folding stabilization we hypothesize that this protein may have less avidity to RNA than it's L. major counterpart but still account for a significant functional activity in the parasite. The model obtained will help in the design of mutagenesis experiments aimed to elucidate the mechanism of gene expression in trypanosomatids and serve as a starting point for its exploration as a potential source of targets for a rational chemotherapy. | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliación | Tropical Medicine Institute. Unit of Leishmania Cloning. Hamburg, Germany. | pt_BR |
| Afiliación | Tropical Medicine Institute. Unit of Leishmania Cloning. Hamburg, Germany. | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Bioquímica de Proteínas e Peptídeos Rio de Janeiro, RJ, Brasil. | pt_BR |
| Palavras clave en Inglês | Molecular modeling | pt_BR |
| Palavras clave en Inglês | Poly-A binding protein | pt_BR |
| Palavras clave en Inglês | Leishmania amazonensis | pt_BR |
| Palavras clave en Inglês | LaPABP | pt_BR |
| Palavras clave en Inglês | Bioinformatics | pt_BR |
| e-ISSN | 1678-8060 |
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