| Author | Cuervo, Patrícia | |
| Author | Cupolillo, Elisa | |
| Author | Segura, Iris | |
| Author | Saravia, Nancy | |
| Author | Fernandes, Octavio | |
| Access date | 2020-10-26T18:56:13Z | |
| Available date | 2020-10-26T18:56:13Z | |
| Document date | 2002 | |
| Citation | CUERVO, Patricia et al. Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n. 6, p. 877-880, Sept. 2002. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44209 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Espaçadores transcritos internos | pt_BR |
| Subject in Portuguese | Mini-exon gene | pt_BR |
| Subject in Portuguese | DNA ribossômico | pt_BR |
| Subject in Portuguese | Colômbia | pt_BR |
| Title | Genetic Diversity of Colombian Sylvatic Trypanosoma cruzi Isolates Revealed by the Ribosomal DNA | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762002000600023 | |
| Abstract | American trypanosomiasis is a common zoonosis in Colombia and Trypanosoma cruzi presents a wide distribution throughout the country. Although some studies based on enzyme electrophoresis profiles have described the
population structure of the parasite, very few molecular analyses of genotipic markers have been conducted using
Colombian strains. In this study, we amplified the non-transcribed spacer of the mini-gene by PCR, typing the
isolates as T. cruzi I, T. cruzi zymodeme 3 or T. rangeli. In addition, the internal transcribed spacers of the ribosomal
gene concomitant with the 5.8S rDNA were amplified and submitted to restriction fragment polymorphism analysis.
The profiles were analyzed by a numerical methodology generating a phenetic dendrogram that shows heterogeneity among the T. cruzi isolates. This finding suggests a relationship between the complexity of the sylvatic transmission cycle in Colombia and the diversity of the sylvan parasites. | pt_BR |
| Affilliation | Centro Internacional de Entrenamiento e Investigaciones Médicas. Cali, Colombia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Centro Internacional de Entrenamiento e Investigaciones Médicas. Cali, Colombia. | pt_BR |
| Affilliation | Centro Internacional de Entrenamiento e Investigaciones Médicas. Cali, Colombia. | pt_BR |
| Affilliation | Universidade do Estado de Rio de Janeiro. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Internal transcribed spacers | pt_BR |
| Subject | Mini-exon gene | pt_BR |
| Subject | Ribosomal DNA | pt_BR |
| Subject | Colombia | pt_BR |
| e-ISSN | 1678-8060 | |
