Author | Thaumaturgo, Nilton | |
Author | Vilar, Mônica Magno | |
Author | Edelenyi, Ricardo | |
Author | Tendler, Miriam | |
Access date | 2020-10-27T18:54:34Z | |
Available date | 2020-10-27T18:54:34Z | |
Document date | 2002 | |
Citation | THAUMATURGO, Nilton et al. Characterization of Sm14 Related Components in Different Helminths by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Western Blotting Analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, Suppl.1, p. 115-116, 2002. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44237 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Sm14 | pt_BR |
Subject in Portuguese | Schistosoma mansoni | pt_BR |
Subject in Portuguese | Reação cruzada imunológica | pt_BR |
Subject in Portuguese | Proteína de ligação de ácidos graxos | pt_BR |
Title | Characterization of Sm14 Related Components in Different Helminths by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Western Blotting Analysis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1590/S0074-02762002000900024 | |
Abstract | Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same
family were identified in several helminth species, with high aminoacid sequence homology between them. In
addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously
immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. In the present study, data on preliminary
sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts – Ascaris
suum (males and females), Echinostoma paraensei, and Taenia saginata – presented components that comigrated
with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Sm14 | pt_BR |
Subject | Schistosoma mansoni | pt_BR |
Subject | Immune crossreaction | pt_BR |
Subject | Fatty acid-binding protein | pt_BR |
e-ISSN | 1678-8060 | |