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| Autor | Thaumaturgo, Nilton | |
| Autor | Vilar, Mônica Magno | |
| Autor | Edelenyi, Ricardo | |
| Autor | Tendler, Miriam | |
| Fecha de acceso | 2020-10-27T18:54:34Z | |
| Fecha de disponibilización | 2020-10-27T18:54:34Z | |
| Fecha de publicación | 2002 | |
| Referencia | THAUMATURGO, Nilton et al. Characterization of Sm14 Related Components in Different Helminths by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Western Blotting Analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, Suppl.1, p. 115-116, 2002. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/44237 | |
| Idioma | eng | pt_BR |
| Editor | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
| Derechos de autor | open access | |
| Palabras clave en Portugués | Sm14 | pt_BR |
| Palabras clave en Portugués | Schistosoma mansoni | pt_BR |
| Palabras clave en Portugués | Reação cruzada imunológica | pt_BR |
| Palabras clave en Portugués | Proteína de ligação de ácidos graxos | pt_BR |
| Título | Characterization of Sm14 Related Components in Different Helminths by Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis and Western Blotting Analysis | pt_BR |
| Tipo del documento | Article | |
| DOI | 10.1590/S0074-02762002000900024 | |
| Resumen en Inglés | Sm14 was the first fatty acid-binding protein homologue identified in helminths. Thereafter, members of the same family were identified in several helminth species, with high aminoacid sequence homology between them. In addition, immune crossprotection was also reported against Fasciola hepatica infection, in animals previously immunized with the Schistosoma mansoni vaccine candidate, r-Sm14. In the present study, data on preliminary sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis of nine different helminth extracts focusing the identification of Sm14 related proteins, is reported. Out of these, three extracts – Ascaris suum (males and females), Echinostoma paraensei, and Taenia saginata – presented components that comigrated with Sm14 in SDS-PAGE, and that were recognized by anti-rSm14 policlonal serum, in Western blotting tests. | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Afiliación | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Palavras clave en Inglês | Sm14 | pt_BR |
| Palavras clave en Inglês | Schistosoma mansoni | pt_BR |
| Palavras clave en Inglês | Immune crossreaction | pt_BR |
| Palavras clave en Inglês | Fatty acid-binding protein | pt_BR |
| e-ISSN | 1678-8060 |
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